LOCUS       BBa_I732738             1254 bp    DNA     linear       19-May-2024
DEFINITION  P_D01O1wt1 + Double Reporters (lacZ+RFP)
ACCESSION   BBa_I732738
VERSION     BBa_I732738.1  
FEATURES             Location/Qualifiers
     promoter        1..125
                     /label=BBa_I732200
     misc_feature    138..141
                     /label=conserved
     RBS             134..145
                     /label=BBa_B0034
     CDS             152..385
                     /label=lacZ
     misc_feature    152..154
                     /label=START
     misc_feature    380..385
                     /label=STOP
     CDS             152..385
                     /label=BBa_I732006
     misc_feature    134..385
                     /label=BBa_I732018
     misc_feature    398..401
                     /label=conserved
     RBS             394..405
                     /label=BBa_B0034
     CDS             412..1086
                     /label=mrfp1
     misc_feature    1093..1117
                     /label=Help:Barcodes
     CDS             412..1117
                     /label=BBa_E1010
     stem_loop       1137..1180
                     /label=stem_loop
     misc_feature    1126..1205
                     /label=BBa_B0010
     terminator      1126..1205
                     /label=BBa_B0010
     stem_loop       1221..1240
                     /label=T7 TE
     misc_feature    1247..1247
                     /label=stop
     polyA_site      1241..1254
                     /label=polya
     misc_feature    1214..1254
                     /label=BBa_B0012
     terminator      1214..1254
                     /label=BBa_B0012
     terminator      1126..1254
                     /label=BBa_B0015
     misc_feature    134..1254
                     /label=BBa_I732092
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaattgga
      121 tccggtacta gagaaagagg agaaatacta gatgaccatg attacggatt cactggccgt
      181 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      241 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      301 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
      361 gccggaaagc tggctggagt aataatacta gagaaagagg agaaatacta gatggcttcc
      421 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      481 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      541 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      601 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      661 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      721 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      781 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      841 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
      901 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
      961 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1021 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1081 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
     1141 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1201 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//