LOCUS       BBa_I732747             1290 bp    DNA     linear       19-May-2024
DEFINITION  P_U073O27+D062NUL + Double Reporters (lacZa+RFP)
ACCESSION   BBa_I732747
VERSION     BBa_I732747.1  
FEATURES             Location/Qualifiers
     promoter        1..161
                     /label=BBa_I732451
     misc_feature    174..177
                     /label=conserved
     RBS             170..181
                     /label=BBa_B0034
     CDS             188..421
                     /label=lacZ
     misc_feature    188..190
                     /label=START
     misc_feature    416..421
                     /label=STOP
     CDS             188..421
                     /label=BBa_I732006
     misc_feature    170..421
                     /label=BBa_I732018
     misc_feature    434..437
                     /label=conserved
     RBS             430..441
                     /label=BBa_B0034
     CDS             448..1122
                     /label=mrfp1
     misc_feature    1129..1153
                     /label=Help:Barcodes
     CDS             448..1153
                     /label=BBa_E1010
     stem_loop       1173..1216
                     /label=stem_loop
     misc_feature    1162..1241
                     /label=BBa_B0010
     terminator      1162..1241
                     /label=BBa_B0010
     stem_loop       1257..1276
                     /label=T7 TE
     misc_feature    1283..1283
                     /label=stop
     polyA_site      1277..1290
                     /label=polya
     misc_feature    1250..1290
                     /label=BBa_B0012
     terminator      1250..1290
                     /label=BBa_B0012
     terminator      1162..1290
                     /label=BBa_B0015
     misc_feature    170..1290
                     /label=BBa_I732092
ORIGIN
        1 aattgtaagc tacagtcgtc gaactcgatt gtatctgtag tgctttagta gtggagttta
       61 cactttatgc ttccggctcg tataatgtgt ggaacatccc ctttatctat cgcctgtgcc
      121 cactacatca agccaaatta aacaggatta acaggatccg gtactagaga aagaggagaa
      181 atactagatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga
      241 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      361 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtaata
      421 atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc
      481 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt
      541 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt
      601 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct
      661 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc
      721 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc
      781 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc
      841 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac
      901 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt
      961 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt
     1021 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
     1081 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc
     1141 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1201 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1261 caccttcggg tgggcctttc tgcgtttata
//