LOCUS       BBa_I732753             1263 bp    DNA     linear       17-May-2024
DEFINITION  P_U073O22+D001O47 + Double Reporters
ACCESSION   BBa_I732753
VERSION     BBa_I732753.1  
FEATURES             Location/Qualifiers
     promoter        1..120
                     /label=BBa_I732304
     misc_feature    133..136
                     /label=conserved
     RBS             129..140
                     /label=BBa_B0034
     CDS             147..380
                     /label=lacZ
     misc_feature    147..149
                     /label=START
     misc_feature    375..380
                     /label=STOP
     CDS             147..380
                     /label=BBa_I732006
     misc_feature    393..396
                     /label=conserved
     RBS             389..400
                     /label=BBa_B0034
     CDS             407..1126
                     /label=GFP protein
     CDS             407..1126
                     /label=BBa_E0040
     stem_loop       1146..1189
                     /label=stem_loop
     misc_feature    1135..1214
                     /label=BBa_B0010
     terminator      1135..1214
                     /label=BBa_B0010
     stem_loop       1230..1249
                     /label=T7 TE
     misc_feature    1256..1256
                     /label=stop
     polyA_site      1250..1263
                     /label=polya
     misc_feature    1223..1263
                     /label=BBa_B0012
     terminator      1223..1263
                     /label=BBa_B0012
ORIGIN
        1 aattgtaagc gcttacaatt gaactcgatt gtatctgtag tgctttagta gtggagttta
       61 cactttatgc ttccggctcg tataatgtgt ggaattgtga actacagtcg tcggatccgg
      121 tactagagaa agaggagaaa tactagatga ccatgattac ggattcactg gccgtcgttt
      181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      301 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
      361 aaagctggct ggagtaataa tactagagaa agaggagaaa tactagatgc gtaaaggaga
      421 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      481 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      541 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      601 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      661 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      721 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      781 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      841 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      901 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      961 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
     1021 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     1081 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
     1141 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1201 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1261 ata
//