LOCUS       BBa_I732754             1264 bp    DNA     linear       17-May-2024
DEFINITION  P_U073O11+D002O22 + Double Reporters
ACCESSION   BBa_I732754
VERSION     BBa_I732754.1  
FEATURES             Location/Qualifiers
     promoter        1..121
                     /label=BBa_I732306
     misc_feature    134..137
                     /label=conserved
     RBS             130..141
                     /label=BBa_B0034
     CDS             148..381
                     /label=lacZ
     misc_feature    148..150
                     /label=START
     misc_feature    376..381
                     /label=STOP
     CDS             148..381
                     /label=BBa_I732006
     misc_feature    394..397
                     /label=conserved
     RBS             390..401
                     /label=BBa_B0034
     CDS             408..1127
                     /label=GFP protein
     CDS             408..1127
                     /label=BBa_E0040
     stem_loop       1147..1190
                     /label=stem_loop
     misc_feature    1136..1215
                     /label=BBa_B0010
     terminator      1136..1215
                     /label=BBa_B0010
     stem_loop       1231..1250
                     /label=T7 TE
     misc_feature    1257..1257
                     /label=stop
     polyA_site      1251..1264
                     /label=polya
     misc_feature    1224..1264
                     /label=BBa_B0012
     terminator      1224..1264
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc gctcacaatt gaactcgatt gtatctgtag tgctttagta gtggagttta
       61 cactttatgc ttccggctcg tataatgtgt ggaaattgta agcgcttaca attggatccg
      121 gtactagaga aagaggagaa atactagatg accatgatta cggattcact ggccgtcgtt
      181 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      241 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      301 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
      361 gaaagctggc tggagtaata atactagaga aagaggagaa atactagatg cgtaaaggag
      421 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      481 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      541 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      601 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      661 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      721 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      781 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      841 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      901 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      961 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1021 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1081 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
     1141 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1201 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1261 tata
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