LOCUS       BBa_I732902              597 bp    DNA     linear       19-May-2024
DEFINITION  R0010 I732020
ACCESSION   BBa_I732902
VERSION     BBa_I732902.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..460
                     /label=lacZ
     misc_feature    227..229
                     /label=START
     misc_feature    455..460
                     /label=STOP
     CDS             227..460
                     /label=BBa_I732006
     stem_loop       480..523
                     /label=stem_loop
     misc_feature    469..548
                     /label=BBa_B0010
     terminator      469..548
                     /label=BBa_B0010
     stem_loop       564..583
                     /label=T7 TE
     misc_feature    590..590
                     /label=stop
     polyA_site      584..597
                     /label=polya
     misc_feature    557..597
                     /label=BBa_B0012
     terminator      557..597
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ccatgattac
      241 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      301 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      361 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
      421 ggcaccagaa gcggtgccgg aaagctggct ggagtaataa tactagagcc aggcatcaaa
      481 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      541 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//