LOCUS BBa_I732922 4301 bp DNA linear 17-May-2024 DEFINITION I732086 I732822 J09855 I732715 ACCESSION BBa_I732922 VERSION BBa_I732922.1 FEATURES Location/Qualifiers misc_feature 1..689 /label=BBa_I732084 stem_loop 709..752 /label=stem_loop misc_feature 698..777 /label=BBa_B0010 terminator 698..777 /label=BBa_B0010 stem_loop 793..812 /label=T7 TE misc_feature 819..819 /label=stop polyA_site 813..826 /label=polya misc_feature 786..826 /label=BBa_B0012 terminator 786..826 /label=BBa_B0012 misc_feature 835..853 /label=TetR 1 misc_feature 835..888 /label=BBa_R0040 promoter 854..859 /label=-35 misc_feature 860..878 /label=TetR 2 promoter 877..882 /label=-10 promoter 835..888 /label=BBa_R0040 misc_feature 1..888 /label=BBa_I732086 misc_feature 901..904 /label=conserved RBS 897..908 /label=BBa_B0034 CDS 915..2000 /label=BBa_I732102 stem_loop 2020..2063 /label=stem_loop misc_feature 2009..2088 /label=BBa_B0010 terminator 2009..2088 /label=BBa_B0010 stem_loop 2104..2123 /label=T7 TE misc_feature 2130..2130 /label=stop polyA_site 2124..2137 /label=polya misc_feature 2097..2137 /label=BBa_B0012 terminator 2097..2137 /label=BBa_B0012 misc_feature 897..2137 /label=BBa_I732822 misc_feature 2146..2182 /label=P(Lac) IQ promoter 2148..2153 /label=-35 promoter 2171..2176 /label=-10 promoter 2146..2182 /label=BBa_I14032 misc_feature 2195..2198 /label=conserved RBS 2191..2202 /label=BBa_B0034 misc_feature 2209..2210 /label=prefix misc_feature 2382..2382 /label=T misc_feature 2700..2700 /label=A CDS 2209..2958 /label=luxR misc_feature 2209..2964 /label=BBa_C0062 misc_feature 2965..2989 /label=Help:Barcodes CDS 2209..2964 /label=BBa_C0062 stem_loop 3009..3052 /label=stem_loop misc_feature 2998..3077 /label=BBa_B0010 terminator 2998..3077 /label=BBa_B0010 stem_loop 3093..3112 /label=T7 TE misc_feature 3119..3119 /label=stop polyA_site 3113..3126 /label=polya misc_feature 3086..3126 /label=BBa_B0012 terminator 3086..3126 /label=BBa_B0012 misc_feature 2191..3126 /label=BBa_I0462 misc_feature 3135..3154 /label=LuxR/HSL promoter 3154..3159 /label=-35 promoter 3176..3181 /label=-10 misc_feature 3187..3187 /label=start misc_feature 3135..3189 /label=BBa_R0062 promoter 3135..3189 /label=BBa_R0062 misc_feature 2146..3189 /label=BBa_J09855 misc_feature 3202..3205 /label=conserved RBS 3198..3209 /label=BBa_B0034 CDS 3216..4301 /label=BBa_I732115 misc_feature 3198..4301 /label=BBa_I732815 ORIGIN 1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact 61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg 121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta 181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt 241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac 301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac 361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt 421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt 481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa 541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc 601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac 661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag 721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta 841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag 901 aggagaaata ctagatgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 961 ctcatcagac cgtttccaac gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 1021 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 1081 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 1141 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 1201 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 1261 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 1321 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 1381 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 1441 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 1501 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 1561 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 1621 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 1681 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 1741 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 1801 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 1861 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 1921 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 1981 tggaaagcgg gcagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2041 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2101 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtggtg caaaaccttt 2161 cgcggtatgg catgatagcg cctactagag aaagaggaga aatactagat gaaaaacata 2221 aatgccgacg acacatacag aataattaat aaaattaaag cttgtagaag caataatgat 2281 attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg 2341 atcatttatc ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa 2401 aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat 2461 tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa 2521 aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc 2581 cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac 2641 aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct 2701 ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc 2761 aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca 2821 aaaatattag gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa 2881 ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat 2941 tgcccatact ttaaaaatta ataacactga tagtgctagt gtagatcact actagagcca 3001 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3061 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3121 tttatatact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag 3181 tcgaataaat actagagaaa gaggagaaat actagatgaa accagtaacg ttatacgatg 3241 tcgcagagta tgccggtgtc tctcgccaaa ccgtttcccg cgtggtgaac caggccagcc 3301 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc 3361 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct 3421 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc 3481 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag 3541 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg 3601 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg 3661 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac 3721 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat 3781 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc 3841 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa 3901 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga 3961 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct 4021 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca 4081 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc 4141 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca 4201 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 4261 tggcacgaca ggtttcccga ctggaaagcg ggcagtaata a //