LOCUS       BBa_I732924             2222 bp    DNA     linear       19-May-2024
DEFINITION  Combined Gates (version 1)
ACCESSION   BBa_I732924
VERSION     BBa_I732924.1  
FEATURES             Location/Qualifiers
     promoter        1..120
                     /label=BBa_I732303
     misc_feature    133..136
                     /label=conserved
     RBS             129..140
                     /label=BBa_B0034
     CDS             147..380
                     /label=lacZ
     misc_feature    147..149
                     /label=START
     misc_feature    375..380
                     /label=STOP
     CDS             147..380
                     /label=BBa_I732006
     misc_feature    393..396
                     /label=conserved
     RBS             389..400
                     /label=BBa_B0034
     CDS             407..1126
                     /label=GFP protein
     CDS             407..1126
                     /label=BBa_E0040
     stem_loop       1146..1189
                     /label=stem_loop
     misc_feature    1135..1214
                     /label=BBa_B0010
     terminator      1135..1214
                     /label=BBa_B0010
     stem_loop       1230..1249
                     /label=T7 TE
     misc_feature    1256..1256
                     /label=stop
     polyA_site      1250..1263
                     /label=polya
     misc_feature    1223..1263
                     /label=BBa_B0012
     terminator      1223..1263
                     /label=BBa_B0012
     misc_feature    1..1263
                     /label=BBa_I732720
     promoter        1272..1353
                     /label=BBa_I732352
     misc_feature    1366..1369
                     /label=conserved
     RBS             1362..1373
                     /label=BBa_B0034
     CDS             1380..2054
                     /label=mrfp1
     misc_feature    2061..2085
                     /label=Help:Barcodes
     CDS             1380..2085
                     /label=BBa_E1010
     stem_loop       2105..2148
                     /label=stem_loop
     misc_feature    2094..2173
                     /label=BBa_B0010
     terminator      2094..2173
                     /label=BBa_B0010
     stem_loop       2189..2208
                     /label=T7 TE
     misc_feature    2215..2215
                     /label=stop
     polyA_site      2209..2222
                     /label=polya
     misc_feature    2182..2222
                     /label=BBa_B0012
     terminator      2182..2222
                     /label=BBa_B0012
     terminator      2094..2222
                     /label=BBa_B0015
     misc_feature    1272..2222
                     /label=BBa_I732917
ORIGIN
        1 aattgtaagc gcttacaatt gaactcgatt gtatctgtag tgctttagta gtggagttta
       61 cactttatgc ttccggctcg tataatgtgt ggaattgtga acgctcataa ttggatccgg
      121 tactagagaa agaggagaaa tactagatga ccatgattac ggattcactg gccgtcgttt
      181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      301 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
      361 aaagctggct ggagtaataa tactagagaa agaggagaaa tactagatgc gtaaaggaga
      421 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      481 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      541 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      601 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      661 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      721 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      781 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      841 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      901 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      961 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
     1021 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     1081 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
     1141 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1201 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1261 atatactaga gaattgtgaa cgttcacaat ttacacttta tgcttccggc tcgtataatg
     1321 tgtggaattg taagcgctta caattggatc cggtactaga gaaagaggag aaatactaga
     1381 tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt
     1441 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta
     1501 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc
     1561 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg
     1621 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag
     1681 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca
     1741 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca
     1801 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa
     1861 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct
     1921 acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca
     1981 tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc
     2041 actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca
     2101 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2161 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2221 ta
//