LOCUS       BBa_I732926             2226 bp    DNA     linear       21-May-2024
DEFINITION  Combined Gates (version 3)
ACCESSION   BBa_I732926
VERSION     BBa_I732926.1  
FEATURES             Location/Qualifiers
     promoter        1..124
                     /label=BBa_I732274
     misc_feature    137..140
                     /label=conserved
     RBS             133..144
                     /label=BBa_B0034
     CDS             151..384
                     /label=lacZ
     misc_feature    151..153
                     /label=START
     misc_feature    379..384
                     /label=STOP
     CDS             151..384
                     /label=BBa_I732006
     misc_feature    397..400
                     /label=conserved
     RBS             393..404
                     /label=BBa_B0034
     CDS             411..1130
                     /label=GFP protein
     CDS             411..1130
                     /label=BBa_E0040
     stem_loop       1150..1193
                     /label=stem_loop
     misc_feature    1139..1218
                     /label=BBa_B0010
     terminator      1139..1218
                     /label=BBa_B0010
     stem_loop       1234..1253
                     /label=T7 TE
     misc_feature    1260..1260
                     /label=stop
     polyA_site      1254..1267
                     /label=polya
     misc_feature    1227..1267
                     /label=BBa_B0012
     terminator      1227..1267
                     /label=BBa_B0012
     misc_feature    1..1267
                     /label=BBa_I732712
     promoter        1276..1357
                     /label=BBa_I732352
     misc_feature    1370..1373
                     /label=conserved
     RBS             1366..1377
                     /label=BBa_B0034
     CDS             1384..2058
                     /label=mrfp1
     misc_feature    2065..2089
                     /label=Help:Barcodes
     CDS             1384..2089
                     /label=BBa_E1010
     stem_loop       2109..2152
                     /label=stem_loop
     misc_feature    2098..2177
                     /label=BBa_B0010
     terminator      2098..2177
                     /label=BBa_B0010
     stem_loop       2193..2212
                     /label=T7 TE
     misc_feature    2219..2219
                     /label=stop
     polyA_site      2213..2226
                     /label=polya
     misc_feature    2186..2226
                     /label=BBa_B0012
     terminator      2186..2226
                     /label=BBa_B0012
     terminator      2098..2226
                     /label=BBa_B0015
     misc_feature    1276..2226
                     /label=BBa_I732917
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtaagcgttc acaattggat
      121 ccggtactag agaaagagga gaaatactag atgaccatga ttacggattc actggccgtc
      181 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      241 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      301 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
      361 ccggaaagct ggctggagta ataatactag agaaagagga gaaatactag atgcgtaaag
      421 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      481 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      541 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      601 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      661 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      721 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      781 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      841 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      901 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      961 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1021 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1081 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
     1141 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1201 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1261 gtttatatac tagagaattg tgaacgttca caatttacac tttatgcttc cggctcgtat
     1321 aatgtgtgga attgtaagcg cttacaattg gatccggtac tagagaaaga ggagaaatac
     1381 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
     1441 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
     1501 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
     1561 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
     1621 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
     1681 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
     1741 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
     1801 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
     1861 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
     1921 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
     1981 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
     2041 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
     2101 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2161 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2221 tttata
//