LOCUS       BBa_I741001             1996 bp    DNA     linear       23-May-2024
DEFINITION  HSL Receiver Construct
ACCESSION   BBa_I741001
VERSION     BBa_I741001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..65
                     /label=prefix
     misc_feature    237..237
                     /label=T
     misc_feature    555..555
                     /label=A
     CDS             64..813
                     /label=luxR
     misc_feature    64..819
                     /label=BBa_C0062
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             64..819
                     /label=BBa_C0062
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     misc_feature    46..981
                     /label=BBa_I0462
     misc_feature    990..1009
                     /label=LuxR/HSL
     promoter        1009..1014
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1042..1042
                     /label=start
     misc_feature    990..1044
                     /label=BBa_R0062
     promoter        990..1044
                     /label=BBa_R0062
     misc_feature    1..1044
                     /label=BBa_J09855
     RBS             1053..1063
                     /label=RBS-4\Weaker
     RBS             1059..1062
                     /label=RBS
     misc_feature    1053..1063
                     /label=BBa_B0033
     RBS             1053..1063
                     /label=BBa_B0033
     CDS             1070..1996
                     /label=BBa_J09271
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag agtcacacag gactactaga tgaagaatca
     1081 agcgcatccg attattgtcg tcaaacgacg caaagccaaa agccacgggg cagcacatgg
     1141 atcgtggaag attgcttatg ccgactttat gactgcgatg atggcctttt ttctggtgat
     1201 gtggctgatc tccatctcca gcccaaaaga gctgattcag attgcggagt acttccggac
     1261 tccactggcg actgcggtta cgggcggcga tcgcatttct aatagtgaaa gcccaattcc
     1321 cggcggtggt gatgattaca cccaaagcca gggggaagtg aataagcagc cgaacatcga
     1381 agagctgaaa aaacgcatgg agcaaagtcg attgcggaaa ttgcggggtg atctcgacca
     1441 gttgatagag tccgatccga aactgcgggc gttacgtccc catctcaaaa tcgatctggt
     1501 ccaggaaggt ctacgtattc agatcatcga tagccagaat cgcccgatgt ttagaaccgg
     1561 cagtgccgat gtcgaaccct atatgcgcga cattctgcgc gccattgcgc ctgtactgaa
     1621 cggtattccc aaccgtatta gcctttcagg tcataccgat gatttcccct acgccagcgg
     1681 tgagaaagga tatagcaact gggagctttc tgccgatcgg gccaatgcat cccgccgcga
     1741 actgatggtc ggagggttgg atagcggcaa agtgttacgt gtcgtcggca tggcggcaac
     1801 gatgcgctta agcgatcgcg gacctgatga tgccgtcaac cgtcgcatca gcctgctggt
     1861 actgaacaaa caagccgaac aggccatttt gcatgaaaac gccgaaagcc agaatgagcc
     1921 agtaagcgcc ctggaaaaac ctgaggttgc accacaggtc agtgttccca caatgccatc
     1981 agccgaaccg aggtga
//