LOCUS       BBa_I742128             1167 bp    DNA     linear       09-May-2024
DEFINITION  Cell replication --> YFP on/off
ACCESSION   BBa_I742128
VERSION     BBa_I742128.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    4..31
                     /label=dif site (forward)
     misc_feature    1..31
                     /label=BBa_I742101
     misc_feature    117..154
                     /label=CAP binding site
     misc_feature    50..70
                     /label=LacI binding site
     promoter        100..105
                     /label=Plac -35
     promoter        77..82
                     /label=Plac -10
     promoter        40..242
                     /label=BBa_I742124
     misc_feature    251..253
                     /label=SacI
     misc_feature    254..281
                     /label=dif site (reverse)
     misc_feature    251..281
                     /label=BBa_I742102
     misc_feature    294..297
                     /label=conserved
     RBS             290..301
                     /label=BBa_B0034
     CDS             308..1030
                     /label=BBa_E0030
     stem_loop       1050..1093
                     /label=stem_loop
     misc_feature    1039..1118
                     /label=BBa_B0010
     terminator      1039..1118
                     /label=BBa_B0010
     stem_loop       1134..1153
                     /label=T7 TE
     misc_feature    1160..1160
                     /label=stop
     polyA_site      1154..1167
                     /label=polya
     misc_feature    1127..1167
                     /label=BBa_B0012
     terminator      1127..1167
                     /label=BBa_B0012
ORIGIN
        1 ctcggtgcgc ataatgtata ttatgttaaa ttactagagc tctgtgtgaa attgttatcc
       61 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      121 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      181 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      241 tgtactagag ctcatttaac ataatataca ttatgcgcac ctactagaga aagaggagaa
      301 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      361 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      421 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      481 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca
      541 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      601 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      661 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      721 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      781 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      841 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      901 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      961 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1021 caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1081 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1141 cttcgggtgg gcctttctgc gtttata
//