LOCUS       BBa_I742132             1775 bp    DNA     linear       08-May-2024
DEFINITION  Division-PoPper: Cell division --> PoPS
ACCESSION   BBa_I742132
VERSION     BBa_I742132.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    46..48
                     /label=SacI
     misc_feature    49..76
                     /label=dif site (forward)
     misc_feature    46..76
                     /label=BBa_I742101
     promoter        85..133
                     /label=BBa_I742126
     misc_feature    146..149
                     /label=conserved
     RBS             142..153
                     /label=BBa_B0034
     CDS             163..779
                     /label=tetR
     misc_feature    780..813
                     /label=SsrA
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             160..844
                     /label=BBa_C0040
     terminator      853..898
                     /label=BBa_I742129
     CDS             910..1656
                     /label=cI repressor coding sequence
     misc_feature    907..1656
                     /label=BBa_I742131
     RBS             1665..1674
                     /label=BBa_I742130
     misc_feature    1683..1701
                     /label=TetR 1
     misc_feature    1683..1736
                     /label=BBa_R0040
     promoter        1702..1707
                     /label=-35
     misc_feature    1708..1726
                     /label=TetR 2
     promoter        1725..1730
                     /label=-10
     promoter        1683..1736
                     /label=BBa_R0040
     misc_feature    1745..1747
                     /label=SacI
     misc_feature    1748..1775
                     /label=dif site (reverse)
     misc_feature    1745..1775
                     /label=BBa_I742102
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagctcgg tgcgcataat
       61 gtatattatg ttaaattact agaggcaacc attatcaccg ccagaggtaa aatagtcaac
      121 acgcacggtg ttatactaga gaaagaggag aaatactaga tgtccagatt agataaaagt
      181 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
      241 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
      301 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
      361 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
      421 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
      481 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
      541 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
      601 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
      661 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
      721 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc
      781 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga
      841 tcactactag agaaataata aaaaagccgg attaataatc tggcttttta tattctctta
      901 ctagagttat taagctacta aagcgtagtt ttcgtcgttt gcagcgccaa acgtctcttc
      961 aggccactga ctagcgataa ctttccccac aacggaacaa ctctcattgc atgggatcat
     1021 tgggtactgt gggtttagtg gttgtaaaaa cacctgaccg ctatccctga tcagtttctt
     1081 gaaggtaaac tcatcacccc caagtctggc tatgcagaaa tcacctggct caacagcctg
     1141 ctcagggtca acgagaatta acattccgtc aggaaagctt ggcttggagc ctgttggtgc
     1201 ggtcatggaa ttaccttcaa cctcaagcca gaatgcagaa tcactggctt ttttggttgt
     1261 gcttacccat ctctccgcat cacctttggt aaaggttcta agctcaggtg agaacatccc
     1321 tgcctgaaca tgagaaaaaa cagggtactc atactcactt ctaagtgacg gctgcatact
     1381 aaccgcttca tacatctcgt agatttctct ggcgattgaa gggctaaatt cttcaacgct
     1441 aactttgaga atttttgcaa gcaatgcggc gttataagca tttaatgcat tgatgccatt
     1501 aaataaagca ccaacgcctg actgccccat ccccatcttg tctgcgacag attcctggga
     1561 taagccaagt tcatttttct ttttttcata aattgcttta aggcgacgtg cgtcctcaag
     1621 ctgctcttgt gttaatggtt tcttttttgt gctcattact agagtctcct cttttactag
     1681 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact
     1741 agagctcatt taacataata tacattatgc gcacc
//