BBa_I742101 1 difF dif site with forward orientation 2007-08-07T11:00:00Z 2015-08-31T04:08:02Z E. coli K12 Released HQ 2013 'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct repeats of the dif site are vital to DNA monomerisation during bacterial septation. As with other recombinase sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enables recombination that is temporally isolated to septation. false false _123_ 0 1965 9 In stock true Constructed as two complimentary oligonucleotides. true Xiaonan Wang annotation1955264 1 dif site (forward) range1955264 1 4 31 annotation1940992 1 SacI range1940992 1 1 3 BBa_I742132 1 BBa_I742132 Division-PoPper: Cell division --> PoPS 2007-10-21T11:00:00Z 2015-08-31T04:08:03Z Please refer to individual parts. They are all more or less modified versions of E. coli K12 loci and its phage lambda. This device gives PoPS-output on cell division. The DNA flanked by the opposing repeats of the dif-site is inverted and allows transcription of both any downstream element and a repressor which, upon sufficient build-up, switches off the promoter which controls downstream transcription. Inversion only occurs when the XerCD complex is activated, and this only occurs at cell division. Therefore this device produces a short burst of PoPS as a function of cell division. Any added downstream elements will likewise be activated, briefly, as a function of cell division. false false _123_ 0 1968 9 Not in stock false Again, please refer to to part in question. However, as a general rule, all parts in 'non-default', bottom DNA strand need be synthesised in reverse complementary fashion. You will encounter parts that are called 'upside-down' or 'reverse complementary' for this reason. false Caroline Dahl component2280656 1 BBa_C0040 component2280661 1 BBa_R0040 component2280657 1 BBa_I742129 component2280646 1 BBa_I14032 component2280649 1 BBa_I742101 component2280659 1 BBa_I742131 component2280660 1 BBa_I742130 component2280650 1 BBa_I742126 component2280668 1 BBa_I742102 component2280652 1 BBa_B0034 annotation2280650 1 BBa_I742126 range2280650 1 85 133 annotation2280646 1 BBa_I14032 range2280646 1 1 37 annotation2280649 1 BBa_I742101 range2280649 1 46 76 annotation2280652 1 BBa_B0034 range2280652 1 142 153 annotation2280656 1 BBa_C0040 range2280656 1 160 844 annotation2280668 1 BBa_I742102 range2280668 1 1745 1775 annotation2280659 1 BBa_I742131 range2280659 1 907 1656 annotation2280660 1 BBa_I742130 range2280660 1 1665 1674 annotation2280657 1 BBa_I742129 range2280657 1 853 898 annotation2280661 1 BBa_R0040 range2280661 1 1683 1736 BBa_I742130 1 revRBS Reverse RBS 2007-10-21T11:00:00Z 2015-08-31T04:08:03Z Please refer to the original (BB_B0034). DNA is double-stranded. To translate genes of both strands, you need RBSs in both strands. false false _123_ 0 1968 9 Not in stock false Please refer to the original (BB_B0034). false Caroline Dahl BBa_C0040 1 tetR tetracycline repressor from transposon Tn10 (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999) Released HQ 2013 Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P> false true _1_ 0 24 7 In stock false References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman. annotation23329 1 tetR range23329 1 4 620 annotation2213989 1 Help:Barcodes range2213989 1 661 685 annotation23330 1 SsrA range23330 1 621 654 BBa_I742126 1 revPlam Reverse lambda cI-regulated promoter 2007-10-21T11:00:00Z 2015-08-31T04:08:02Z See BBa_R0051. This is an exact 'upside down' version of BBa_R0051. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional regulatory potential that it gives. false false _123_ 0 1968 9 Not in stock false Any 'upside down' (reverse complementary) sequences made by PCR amplification need be made with suffix and prefix is opposite order with respect to the original default sequence. false Caroline Dahl BBa_I742102 1 difR dif site with reverse orientation 2007-08-07T11:00:00Z 2015-08-31T04:08:02Z E. coli K12 Released HQ 2013 'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct dif repeats are vital to DNA monomerisation during bacterial septation. As with other recombination sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enable recombination that is temporally isolated to division. false false _123_ 0 1965 9 In stock true Synthesised using two oligonucleotides. true Xiaonan Wang annotation1955265 1 dif site (reverse) range1955265 1 4 31 annotation1940993 1 SacI range1940993 1 1 3 BBa_I14032 1 P(Lac) IQ promoter P(Lac) IQ 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pMAL-p2X Released HQ 2013 Constitutive Promoter, High Transcription false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1028344 1 -10 range1028344 1 26 31 annotation1028342 1 P(Lac) IQ range1028342 1 1 37 annotation1028343 1 -35 range1028343 1 3 8 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986787 1 -10 range1986787 1 43 48 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_I742131 1 rev.cI Inverse cI repressor coding sequence. 2007-10-21T11:00:00Z 2015-08-31T04:08:03Z Please refer to the original, BBa_C0051. This sequence encodes the cl repressor, only it encodes it in the 'non-default', bottom strand, rather than the straight-forward top one. When combined with the cl-responsive promoter, it enables regulatory control of both strands. false false _123_ 0 1968 9 Not in stock false As above. false Caroline Dahl annotation1956553 1 cI repressor coding sequence range1956553 1 4 750 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I742129 1 BBa_I742129 Reverse terminator BBa_B0011 LuxICDABEG (+/-) 2007-10-21T11:00:00Z 2015-08-31T04:08:03Z Please see BBa_B0011 for details. Released HQ 2013 LuxICDABEG (+/-) forms a stem-loop which cancels transcription on both strands, whichever you choose to place it in. Please see BBa_B0011 for details about the terminator. false false _123_ 0 1968 9 In stock false None. false Caroline Dahl BBa_B0034_sequence 1 aaagaggagaaa BBa_I742131_sequence 1 ttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcat BBa_I14032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_I742102_sequence 1 ctcatttaacataatatacattatgcgcacc BBa_C0040_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_I742101_sequence 1 ctcggtgcgcataatgtatattatgttaaat BBa_I742129_sequence 1 aaataataaaaaagccggattaataatctggctttttatattctct BBa_I742132_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagctcggtgcgcataatgtatattatgttaaattactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaataataaaaaagccggattaataatctggctttttatattctcttactagagttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcattactagagtctcctcttttactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagctcatttaacataatatacattatgcgcacc BBa_I742130_sequence 1 tctcctcttt BBa_I742126_sequence 1 gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgtta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z