BBa_I742116 1 BBa_I742116 crtE with native ribosome binding site 2007-10-18T11:00:00Z 2015-08-31T04:08:02Z Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990) Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087 CrtE encodes geranylgeranyl pyrophosphate synthase Enzyme converts two farnesyl diphosphate molecules into geranylgeranyl diphosphate Part of the carotenoid synthesis pathway false true _123_ 0 1964 9 It's complicated false Ensured gene contained ribosome binding site by designing primers to include the site false sarah hollingshead annotation1949816 1 native rbs range1949816 1 1 15 annotation1949804 1 CrtE coding region range1949804 1 16 954 BBa_I742133 1 BBa_I742133 CrtIB genes with PstI site mutated out 2007-10-21T11:00:00Z 2015-08-31T04:08:03Z Genes were both isolated from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080, ATCC 19321 Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990) CrtIB encodes two enzymes, which make up steps three and two in the carotenoid biosynthesis pathway respectively. CrtI encodes phytoene desaturase and CrtB encodes Phytoene synthase. Both cloned genes still have their native ribosome binding sites. More information about the carotenoid synthesis pathway may be found on the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Colour_Production Edinburgh Yoghurt Wiki] false false _123_ 0 1964 9 Not in stock false The CrtI gene contained two PstI sites, which were both mutated out using point mutagenesis false sarah hollingshead annotation1952301 1 CrtB coding region range1952301 1 1498 2430 annotation1952298 1 CrtI coding region range1952298 1 23 1481 annotation1952304 1 CrtB start range1952304 1 1498 1500 annotation1952305 1 CrtI stop range1952305 1 1479 1481 annotation1952302 1 native rbs range1952302 1 1482 1497 annotation1952306 1 CrtB stop range1952306 1 2428 2430 annotation1952297 1 native rbs range1952297 1 1 22 annotation1952303 1 CrtI start range1952303 1 23 25 BBa_I742117 1 BBa_I742117 CrtY with native ribosome binding site 2007-10-18T11:00:00Z 2015-08-31T04:08:02Z from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087 Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990) CrtY encodes a lycopene cyclase Converts lycopene to B-carotene B-carotene is used as an orange pigment and is a vitamin A precursor false true _123_ 0 1964 9 In stock false Ensured amplified gene contained native ribosome binding site false sarah hollingshead annotation1949811 1 native rbs range1949811 1 1 19 annotation1949805 1 crtY range1949805 1 20 1171 BBa_I742118 1 BBa_I742118 CrtZ with native ribosome binding site 2007-10-18T11:00:00Z 2015-08-31T04:08:02Z Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087 Released HQ 2013 crtZ encodes a beta-carotene hydroxylase Converts B-carotene into zeaxanthin (yellow pigment) Part of the carotenoid biosynthesis pathway false true _123_ 0 1964 9 In stock false Ensured amplified gene contained native ribosome binding site false sarah hollingshead annotation1949806 1 CrtZ coding region range1949806 1 17 547 annotation1949810 1 rbs range1949810 1 1 16 BBa_I742139 1 BBa_I742139 Zeaxanthin (yellow pigment) synthesis operon without PstI sites 2007-10-21T11:00:00Z 2015-08-31T04:08:03Z All five genes were isolated from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) More information about carotenoid synthesis may be found in these references: Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990) Zeaaxnthin, a yellow pigment, is synthesised from farnesyl disphosphate in five sequential enzymatic steps. These five enzymes are encoded by the genes CrtE, CrtB, CrtI, CrtY and CrtZ. More information on the zeaxanthin biosynthesis pathway may be found on the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Colour_Production Edinburgh Yoghurt Wiki] false false _123_ 0 1964 9 Not in stock false Two PstI sites have been removed from CrtI gene via point mutation. All five genes were cloned so they retain their native ribosome binding sites. false sarah hollingshead component1952374 1 BBa_I742133 component1952377 1 BBa_I742117 component1952380 1 BBa_I742118 component1952365 1 BBa_I742116 annotation1952377 1 BBa_I742117 range1952377 1 3401 4571 annotation1952365 1 BBa_I742116 range1952365 1 1 954 annotation1952380 1 BBa_I742118 range1952380 1 4580 5126 annotation1952374 1 BBa_I742133 range1952374 1 963 3392 BBa_I742139_sequence 1 ctcgcgttgccgtaaatgtatccgtttataaggacagcccgaatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcatcgttaaagagcgactacatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcttaagtgggagcggctatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataatactagagctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa BBa_I742133_sequence 1 ctcatcgttaaagagcgactacatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa BBa_I742118_sequence 1 ctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa BBa_I742116_sequence 1 ctcgcgttgccgtaaatgtatccgtttataaggacagcccgaatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa BBa_I742117_sequence 1 ctcttaagtgggagcggctatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z