LOCUS       BBa_I746002             1636 bp    DNA     linear       13-May-2024
DEFINITION  AIP generator + mCherry + term
ACCESSION   BBa_I746002
VERSION     BBa_I746002.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=B0034-sequence RBS
     CDS             19..591
                     /label=agrB coding sequence
     RBS             598..609
                     /label=B0034-sequence RBS
     misc_feature    592..597
                     /label=SaII site
     CDS             616..759
                     /label=agrD coding sequence
     misc_feature    1..759
                     /label=BBa_I746000
     misc_feature    772..775
                     /label=conserved
     RBS             768..779
                     /label=BBa_B0034
     CDS             786..1496
                     /label=mCherry
     misc_feature    1137..1137
                     /label=C->T (removing PstI site)
     misc_feature    786..1499
                     /label=BBa_J06504
     stem_loop       1519..1562
                     /label=stem_loop
     misc_feature    1508..1587
                     /label=BBa_B0010
     terminator      1508..1587
                     /label=BBa_B0010
     stem_loop       1603..1622
                     /label=T7 TE
     misc_feature    1629..1629
                     /label=stop
     polyA_site      1623..1636
                     /label=polya
     misc_feature    1596..1636
                     /label=BBa_B0012
     terminator      1596..1636
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaactatttt gacaacaaga tcgaccaatt tgctacttac
       61 ctgcaaaaac gcaacaacct ggaccacatc cagtttctgc aagttcgtct gggcatgcag
      121 gttctggcta aaaacatcgg caaactgatc gttatgtaca ccatcgccta tattctgaac
      181 atcttcctgt ttaccctgat caccaacctg accttctatc tgattcgccg tcatgcccac
      241 ggcgctcatg caccttcttc cttttggtgt tatgtagaga gcattattct gtttatcctg
      301 ctgccgctgg taatcgttaa cttccacatc aatttcctga ttatgatcat cctgactgtg
      361 atttccctgg gtgtaatctc tgtttacgct ccggctgcca ctaagaaaaa gccgattccg
      421 gtgcgcctga tcaaacgtaa aaagtactac gctatcatcg tttccctgac cctgttcatt
      481 atcaccctga ttatcaaaga accgtttgca cagttcatcc agctgggtat tatcatcgaa
      541 gcgatcactc tgctgccgat ctttttcatc aaagaggatc tgaaataata agtcgacaaa
      601 gaggagaaat actagatgaa tactctgttc aatctgtttt tcgatttcat tactggcatc
      661 ctgaagaaca tcggtaacat cgcagcgtac agcacctgcg acttcatcat ggatgaagta
      721 gaggtcccga aagagctgac ccagctgcat gaataataat actagagaaa gaggagaaat
      781 actagatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      841 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      901 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      961 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     1021 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     1081 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc
     1141 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1201 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     1261 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     1321 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     1381 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     1441 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaataat
     1501 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1561 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1621 cctttctgcg tttata
//