LOCUS       BBa_I746107             3047 bp    DNA     linear       07-May-2024
DEFINITION  I746101 (AIP sensor infrastructure) + I746105 (Inducible P2 + GFP 
            reporter)
ACCESSION   BBa_I746107
VERSION     BBa_I746107.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=B0034-sequence RBS
     RBS             1273..1284
                     /label=B0034-sequence RBS
     misc_feature    1267..1272
                     /label=BamHI site
     CDS             19..1266
                     /label=agrC coding sequence
     CDS             1291..1920
                     /label=agrA coding sequence
     misc_feature    1..1920
                     /label=BBa_I746100
     stem_loop       1940..1983
                     /label=stem_loop
     misc_feature    1929..2008
                     /label=BBa_B0010
     terminator      1929..2008
                     /label=BBa_B0010
     stem_loop       2024..2043
                     /label=T7 TE
     misc_feature    2050..2050
                     /label=stop
     polyA_site      2044..2057
                     /label=polya
     misc_feature    2017..2057
                     /label=BBa_B0012
     terminator      2017..2057
                     /label=BBa_B0012
     promoter        2066..2161
                     /label=BBa_I746104
     RBS             2170..2184
                     /label=RBS-1\Strong
     RBS             2177..2181
                     /label=RBS
     misc_feature    2170..2184
                     /label=BBa_B0030
     RBS             2170..2184
                     /label=BBa_B0030
     CDS             2191..2910
                     /label=GFP protein
     CDS             2191..2910
                     /label=BBa_E0040
     stem_loop       2930..2973
                     /label=stem_loop
     misc_feature    2919..2998
                     /label=BBa_B0010
     terminator      2919..2998
                     /label=BBa_B0010
     stem_loop       3014..3033
                     /label=T7 TE
     misc_feature    3040..3040
                     /label=stop
     polyA_site      3034..3047
                     /label=polya
     misc_feature    3007..3047
                     /label=BBa_B0012
     terminator      3007..3047
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gattctgatg ttcaccattc cagcaattat tagcggtatt
       61 aagtacagca agctggatta tttcttcatc atcgtaatct ctaccctgtc cctgttcctg
      121 ttcaaaatgt tcgattccgc gagcctgatt atcctgactt ccttcatcat catcatgtat
      181 ttcgtaaaaa tcaaatggta tagcatcctg ctgatcatga cctcccagat tatcctgtac
      241 tgtgccaact acatgtatat cgttatctac gcgtacatca cgaaaatctc cgattctatc
      301 ttcgttatct ttccgtcttt cttcgtggtg tacgttacga tctctattct gttttcctat
      361 attatcaatc gcgttctgaa aaagatctct actccgtacc tgattctgaa caaaggtttt
      421 ctgatcgtca tctccactat cctgctgctg accttctctc tgttcttctt ttactctcag
      481 atcaactccg atgaggcaaa agtcatccgt cagtacagct tcatctttat cggtattact
      541 atcttcctga gcattctgac tttcgttatc agccagttcc tgctgaaaga aatgaagtac
      601 aagcgcaacc aagaagaaat cgagacttac tacgagtaca ccctgaagat tgaagcgatc
      661 aacaacgaaa tgcgtaaatt tcgtcacgac tacgttaaca ttctgaccac cctgagcgaa
      721 tacatccgtg aagacgacat gcctggcctg cgtgattatt tcaacaaaaa cattgtgccg
      781 atgaaagata acctgcaaat gaacgctatt aagctgaacg gtatcgaaaa cctgaaagta
      841 cgtgagatca agggtctgat taccgctaaa atcctgcgtg cgcaggaaat gaacatccca
      901 attagcatcg aaatcccaga cgaagtttct agcattaacc tgaacatgat tgacctgtct
      961 cgtagcatcg gcatcatcct ggacaacgct atcgaagctt ccactgaaat cgacgacccg
     1021 attattcgcg ttgcttttat cgagagcgaa aactccgtga cctttatcgt gatgaacaaa
     1081 tgcgcggacg atatcccgcg tattcacgaa ctgttccagg aatctttcag caccaagggt
     1141 gaaggtcgcg gcctgggcct gtccaccctg aaagagatcg cggataacgc cgacaacgtt
     1201 ctgctggaca ctattatcga aaacggtttc ttcattcaga aagtcgaaat catcaataac
     1261 taataaggat ccaaagagga gaaatactag atggaaatcg cactggctac ggacaatcca
     1321 tatgaggttc tggaacaggc taaaaacatg aacgacatcg gctgctattt cctggatatt
     1381 cagctgagca ccgacatcaa cggtatcaaa ctgggctctg aaatccgtaa acatgatccg
     1441 gttggtaaca ttattttcgt gacttctcat tctgagctga cctatctgac gttcgtatac
     1501 aaggttgcag ccatggattt catctttaaa gacgatccgg cagagctgcg tacgcgcatc
     1561 atcgactgtc tggaaactgc gcacacgcgc ctgcaactgc tgtccaaaga taactccgtg
     1621 gaaaccattg aactgaaacg tggcagcaac agcgtttacg ttcagtacga tgatatcatg
     1681 ttcttcgaaa gctccactaa gtcccaccgt ctgatcgcgc atctggataa tcgtcagatc
     1741 gagttctatg gcaacctgaa agagctgagc cagctggacg atcgcttctt ccgttgtcat
     1801 aactccttcg ttgttaaccg tcacaacatc gaatccatcg actccaaaga gcgtattgta
     1861 tacttcaaga acaaagaaca ctgttatgcg tctgtacgca atgtcaagaa aatctaataa
     1921 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2041 gcctttctgc gtttatatac tagagattaa atacaaatta catttaacag ttaagtattt
     2101 atttcctaca gttaggcaat ataatgataa aagattgtac taaatcgtat aatgacagtg
     2161 atactagaga ttaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
     2221 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     2281 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
     2341 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
     2401 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     2461 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     2521 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     2581 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     2641 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     2701 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     2761 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     2821 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     2881 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     2941 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     3001 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//