Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Narin Hengrung
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:04
AIP Sender
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_I746210_sequence (Version 1) |
Description
This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.
In this part, the AIP generating part I746001 is combined with the permeability device, I746201, so that the AIP produced is able to leave the periplasm of the cell.
Notes
...Source
The AIP generating parts were synthesised by DNA 2.0The permeability device uses a mutant protein supplied by Dr P.E. Klebba.
| Access | Instance | Definition |
| public public public public public public public public public | BBa_J23115 BBa_I746000 BBa_B0010 BBa_B0012 BBa_J23111 BBa_B0034 BBa_I746200 BBa_B0010 BBa_B0012 | BBa_J23115 BBa_I746000 BBa_B0010 BBa_B0012 BBa_J23111 BBa_B0034 BBa_I746200 BBa_B0010 BBa_B0012 |