Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Wyatt
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:04
AIP Sender
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_I746211_sequence (Version 1) |
Description
This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.
In this part, the AIP generating part I746001 is combined with the permeability device, I746201, so that the AIP produced is able to leave the periplasm of the cell.
Notes
N/ASource
Standard assembly.| Access | Instance | Definition |
| public public public public public public public public public | BBa_J23115 BBa_I746000 BBa_B0010 BBa_B0012 BBa_J23115 BBa_B0034 BBa_I746200 BBa_B0010 BBa_B0012 | BBa_J23115 BBa_I746000 BBa_B0010 BBa_B0012 BBa_J23115 BBa_B0034 BBa_I746200 BBa_B0010 BBa_B0012 |