LOCUS BBa_I746220 5504 bp DNA linear 29-Apr-2024 DEFINITION AIP Detector ACCESSION BBa_I746220 VERSION BBa_I746220.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23107 RBS 44..55 /label=B0034-sequence RBS RBS 1316..1327 /label=B0034-sequence RBS misc_feature 1310..1315 /label=BamHI site CDS 62..1309 /label=agrC coding sequence CDS 1334..1963 /label=agrA coding sequence misc_feature 44..1963 /label=BBa_I746100 stem_loop 1983..2026 /label=stem_loop misc_feature 1972..2051 /label=BBa_B0010 terminator 1972..2051 /label=BBa_B0010 stem_loop 2067..2086 /label=T7 TE misc_feature 2093..2093 /label=stop polyA_site 2087..2100 /label=polya misc_feature 2060..2100 /label=BBa_B0012 terminator 2060..2100 /label=BBa_B0012 promoter 2109..2204 /label=BBa_I746104 RBS 2213..2227 /label=RBS-1\Strong RBS 2220..2224 /label=RBS misc_feature 2213..2227 /label=BBa_B0030 RBS 2213..2227 /label=BBa_B0030 CDS 2234..2953 /label=GFP protein CDS 2234..2953 /label=BBa_E0040 stem_loop 2973..3016 /label=stem_loop misc_feature 2962..3041 /label=BBa_B0010 terminator 2962..3041 /label=BBa_B0010 stem_loop 3057..3076 /label=T7 TE misc_feature 3083..3083 /label=stop polyA_site 3077..3090 /label=polya misc_feature 3050..3090 /label=BBa_B0012 terminator 3050..3090 /label=BBa_B0012 promoter 3099..3133 /label=BBa_J23111 misc_feature 3146..3149 /label=conserved RBS 3142..3153 /label=BBa_B0034 CDS 3160..5367 /label=BBa_I746200 stem_loop 5387..5430 /label=stem_loop misc_feature 5376..5455 /label=BBa_B0010 terminator 5376..5455 /label=BBa_B0010 stem_loop 5471..5490 /label=T7 TE misc_feature 5497..5497 /label=stop polyA_site 5491..5504 /label=polya misc_feature 5464..5504 /label=BBa_B0012 terminator 5464..5504 /label=BBa_B0012 ORIGIN 1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgattctg atgttcacca ttccagcaat tattagcggt attaagtaca gcaagctgga 121 ttatttcttc atcatcgtaa tctctaccct gtccctgttc ctgttcaaaa tgttcgattc 181 cgcgagcctg attatcctga cttccttcat catcatcatg tatttcgtaa aaatcaaatg 241 gtatagcatc ctgctgatca tgacctccca gattatcctg tactgtgcca actacatgta 301 tatcgttatc tacgcgtaca tcacgaaaat ctccgattct atcttcgtta tctttccgtc 361 tttcttcgtg gtgtacgtta cgatctctat tctgttttcc tatattatca atcgcgttct 421 gaaaaagatc tctactccgt acctgattct gaacaaaggt tttctgatcg tcatctccac 481 tatcctgctg ctgaccttct ctctgttctt cttttactct cagatcaact ccgatgaggc 541 aaaagtcatc cgtcagtaca gcttcatctt tatcggtatt actatcttcc tgagcattct 601 gactttcgtt atcagccagt tcctgctgaa agaaatgaag tacaagcgca accaagaaga 661 aatcgagact tactacgagt acaccctgaa gattgaagcg atcaacaacg aaatgcgtaa 721 atttcgtcac gactacgtta acattctgac caccctgagc gaatacatcc gtgaagacga 781 catgcctggc ctgcgtgatt atttcaacaa aaacattgtg ccgatgaaag ataacctgca 841 aatgaacgct attaagctga acggtatcga aaacctgaaa gtacgtgaga tcaagggtct 901 gattaccgct aaaatcctgc gtgcgcagga aatgaacatc ccaattagca tcgaaatccc 961 agacgaagtt tctagcatta acctgaacat gattgacctg tctcgtagca tcggcatcat 1021 cctggacaac gctatcgaag cttccactga aatcgacgac ccgattattc gcgttgcttt 1081 tatcgagagc gaaaactccg tgacctttat cgtgatgaac aaatgcgcgg acgatatccc 1141 gcgtattcac gaactgttcc aggaatcttt cagcaccaag ggtgaaggtc gcggcctggg 1201 cctgtccacc ctgaaagaga tcgcggataa cgccgacaac gttctgctgg acactattat 1261 cgaaaacggt ttcttcattc agaaagtcga aatcatcaat aactaataag gatccaaaga 1321 ggagaaatac tagatggaaa tcgcactggc tacggacaat ccatatgagg ttctggaaca 1381 ggctaaaaac atgaacgaca tcggctgcta tttcctggat attcagctga gcaccgacat 1441 caacggtatc aaactgggct ctgaaatccg taaacatgat ccggttggta acattatttt 1501 cgtgacttct cattctgagc tgacctatct gacgttcgta tacaaggttg cagccatgga 1561 tttcatcttt aaagacgatc cggcagagct gcgtacgcgc atcatcgact gtctggaaac 1621 tgcgcacacg cgcctgcaac tgctgtccaa agataactcc gtggaaacca ttgaactgaa 1681 acgtggcagc aacagcgttt acgttcagta cgatgatatc atgttcttcg aaagctccac 1741 taagtcccac cgtctgatcg cgcatctgga taatcgtcag atcgagttct atggcaacct 1801 gaaagagctg agccagctgg acgatcgctt cttccgttgt cataactcct tcgttgttaa 1861 ccgtcacaac atcgaatcca tcgactccaa agagcgtatt gtatacttca agaacaaaga 1921 acactgttat gcgtctgtac gcaatgtcaa gaaaatctaa taatactaga gccaggcatc 1981 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2041 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 2101 tactagagat taaatacaaa ttacatttaa cagttaagta tttatttcct acagttaggc 2161 aatataatga taaaagattg tactaaatcg tataatgaca gtgatactag agattaaaga 2221 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 2281 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 2341 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 2401 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 2461 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 2521 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 2581 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 2641 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 2701 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 2761 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 2821 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 2881 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 2941 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3001 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3061 caccttcggg tgggcctttc tgcgtttata tactagagtt gacggctagc tcagtcctag 3121 gtatagtgct agctactaga gaaagaggag aaatactaga tgaacaagaa gattcattcc 3181 ctggccttgt tggtcaatct ggggatttat ggggtagcgc aggcacaaga gccgaccgat 3241 actcctgttt cacatgacga tactattgtc gttaccgccg ccgagcagaa cttacaggcg 3301 cctggcgttt cgaccatcac cgcagatgaa atccgcaaaa acccggttgc ccgcgatgtg 3361 tcgaagatca tccgtaccat gccaggcgtt aacctgaccg gtaactccac cagtggtcag 3421 cgtgggaata accgacagat tgatattcgc ggtatgggtc cggaaaacac gctgattttg 3481 attgacggca agccggtaag cagccgtaac tcggtgcgtc agggctggcg tggcgagcgc 3541 gatacccgtg gtgatacttc ctgggtgcca cctgaaatga ttgaacgtat tgaagttctg 3601 cgtggtccgg cagctgcgcg ttatggcaac ggcgcggcgg gcggcgtggt taacatcatt 3661 accaaaaaag gcagcggcga gtggcacggc tcctgggacg catatttcaa tgcgccagaa 3721 cataaagagg aaggtgccac caaacgcact aactttagcc tgaccggtcc gctgggcgac 3781 gagttcagct tccgtttgta tggcaacctc gacaaaaccc aggctgacgc gtgggatatc 3841 aaccagggcc atcagtccgc gcgtgccgga acgtatgcca cgacgttacc agccgggcgc 3901 gaaggggtaa tcaacaaaga tattaatggc gtggtgcgct gggatttcgc gccattgcaa 3961 tcgctggaac tggaagcagg ttacagccgc cagggtaacc tgtatgcggg cgacacccag 4021 aataccaact ccgattccta tacccgctcg aaatatggcg atgaaaccaa ccgtctgtat 4081 cgccagaact acgcgctgac ctggaacggt ggctgggata acggcgtgac caccagcaac 4141 tgggtgcagt acgaacacac ccgtaactcg cgtattccgg aaggtctggc gggcggtacc 4201 gaagggaaat ttaacgaaaa agcgacacag gatttcgtcg atatcgatct tgatgacgtg 4261 atgctgcaca gcgaagttaa cctgccgatt gatttcctcg ttaaccagac gctgacgctg 4321 ggtacggagt ggaatcagca acggatgaag gacttaagtt ccaacaccca ggcactgacc 4381 ggaacgaata ccggtggcgc tattgatggc gtgagtacca ccgaccgtag cccgtattca 4441 aaagcagaaa ttttctcgct gtttgccgaa aacaacatgg agctgactga cagcaccatc 4501 gtaacgccgg ggctgcgttt cgatcatcac agtattgtcg gcaataactg gagcccggcg 4561 ctgaacatat cgcaaggttt aggcgatgac ttcacgctga aaatgggcat cgcccgtgct 4621 tataaagcgc cgagcctgta ccagactaac ccgaactaca ttctctacag taaaggtcag 4681 ggttgctatg ccagcgcggg cggctgctat ctgcaaggta acgatgacct gaaagcagaa 4741 accagcatca acaaagagat tggtctggag ttcaaacgcg acgggtggct ggcgggcgtc 4801 acctggttcc gtaacgatta tcgcaataag attgaagcgg gctatgtggc tgtagggcaa 4861 aattgtgaaa cagcggtggt tgaaggtctg gaaggatcgt taaacgtacc ggttagcgaa 4921 acggtgatgt ggaccaataa catcacttat atgctgaaga gtgaaaacaa aaccacgggc 4981 gaccgtttgt cgatcatccc ggagtatacg ttgaactcaa cgctgagctg gcaggcacgg 5041 gaagatttgt cgatgcaaac gaccttcacc tggtacggca agcagcagcc gaagaagtac 5101 aactataaag gtcagccagc ggttggaccg gaaaccaaag aaattagtcc ttacagcatt 5161 gttggcctga gcgcgacctg ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac 5221 aatctgttcg acaaacgttt gtggcgtgcg ggtaatgccc agaccacggg cgatttggca 5281 ggggccaact atatcgccgg tgccggggcg tatacctata acgagccggg acgtacgtgg 5341 tatatgagcg taaacaccca cttctgatac tagagccagg catcaaataa aacgaaaggc 5401 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 5461 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //