LOCUS       BBa_I759003             1763 bp    DNA     linear       19-Apr-2024
DEFINITION  tet-regulated Q (Weak tetR promoter)
ACCESSION   BBa_I759003
VERSION     BBa_I759003.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     RBS             55..111
                     /label=Spacer/RBS
     CDS             113..736
                     /label=Q
     misc_feature    1..735
                     /label=BBa_I759000
     misc_feature    1..735
                     /label=BBa_I759000
     stem_loop       755..798
                     /label=stem_loop
     misc_feature    744..823
                     /label=BBa_B0010
     terminator      744..823
                     /label=BBa_B0010
     stem_loop       839..858
                     /label=T7 TE
     misc_feature    865..865
                     /label=stop
     polyA_site      859..872
                     /label=polya
     misc_feature    832..872
                     /label=BBa_B0012
     terminator      832..872
                     /label=BBa_B0012
     terminator      744..872
                     /label=BBa_B0015
     promoter        881..915
                     /label=BBa_J23116
     misc_feature    928..931
                     /label=conserved
     RBS             924..935
                     /label=BBa_B0034
     CDS             945..1561
                     /label=tetR
     misc_feature    1562..1595
                     /label=SsrA
     misc_feature    1602..1626
                     /label=Help:Barcodes
     CDS             942..1601
                     /label=BBa_C0040
     stem_loop       1646..1689
                     /label=stem_loop
     misc_feature    1635..1714
                     /label=BBa_B0010
     terminator      1635..1714
                     /label=BBa_B0010
     stem_loop       1730..1749
                     /label=T7 TE
     misc_feature    1756..1756
                     /label=stop
     polyA_site      1750..1763
                     /label=polya
     misc_feature    1723..1763
                     /label=BBa_B0012
     terminator      1723..1763
                     /label=BBa_B0012
     misc_feature    924..1763
                     /label=BBa_P0440
     misc_feature    881..1763
                     /label=BBa_S03708
     misc_feature    744..1763
                     /label=BBa_S03724
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 aattcactga gtcatatgat cgatttgggt attaaagagg agaaacaatt gatgagactc
      121 gaaagcgtag ctaaatttca ttcgccaaaa agcccgatga tgagcgactc accacgggcc
      181 acggcttctg actctctttc cggtactgat gtgatggctg ctatggggat ggcgcaatca
      241 caagccggat tcggtatggc tgcattctgc ggtaagcacg aactcagcca gaacgacaaa
      301 caaaaggcta tcaactatct gatgcaattt gcacacaagg tatcggggaa ataccgtggt
      361 gtggcaaagc ttgaaggaaa tactaaggca aaggtactgc aagtgctcgc aacattcgct
      421 tatgcggatt attgccgtag tgccgcgacg ccgggggcaa gatgcagaga ttgccatggt
      481 acaggccgtg cggttgatat tgccaaaaca gagctgtggg ggagagttgt cgagaaagag
      541 tgcggaagat gcaaaggcgt cggctattca aggatgccag caagcgcagc atatcgcgct
      601 gtgacgatgc taatcccaaa ccttacccaa cccacctggt cacgcactgt taagccgctg
      661 tatgacgctc tggtggtgca atgccacaaa gaagagtcaa tcgcagacaa cattttgaat
      721 gcggtcacac gttagtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      781 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      841 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacagcta gctcagtcct
      901 agggactatg ctagctacta gagaaagagg agaaatacta gatgtccaga ttagataaaa
      961 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
     1021 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
     1081 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
     1141 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
     1201 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
     1261 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
     1321 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
     1381 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
     1441 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
     1501 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
     1561 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta
     1621 gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1681 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1741 gggtgggcct ttctgcgttt ata
//