LOCUS       BBa_I759009             1862 bp    DNA     linear       29-Apr-2024
DEFINITION  tet-regulated YFP (Weak tetR promoter)
ACCESSION   BBa_I759009
VERSION     BBa_I759009.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     misc_feature    112..834
                     /label=YFP
     misc_feature    55..111
                     /label=spacer
     misc_feature    1..834
                     /label=BBa_I759008
     stem_loop       854..897
                     /label=stem_loop
     misc_feature    843..922
                     /label=BBa_B0010
     terminator      843..922
                     /label=BBa_B0010
     stem_loop       938..957
                     /label=T7 TE
     misc_feature    964..964
                     /label=stop
     polyA_site      958..971
                     /label=polya
     misc_feature    931..971
                     /label=BBa_B0012
     terminator      931..971
                     /label=BBa_B0012
     terminator      843..971
                     /label=BBa_B0015
     promoter        980..1014
                     /label=BBa_J23116
     misc_feature    1027..1030
                     /label=conserved
     RBS             1023..1034
                     /label=BBa_B0034
     CDS             1044..1660
                     /label=tetR
     misc_feature    1661..1694
                     /label=SsrA
     misc_feature    1701..1725
                     /label=Help:Barcodes
     CDS             1041..1700
                     /label=BBa_C0040
     stem_loop       1745..1788
                     /label=stem_loop
     misc_feature    1734..1813
                     /label=BBa_B0010
     terminator      1734..1813
                     /label=BBa_B0010
     stem_loop       1829..1848
                     /label=T7 TE
     misc_feature    1855..1855
                     /label=stop
     polyA_site      1849..1862
                     /label=polya
     misc_feature    1822..1862
                     /label=BBa_B0012
     terminator      1822..1862
                     /label=BBa_B0012
     misc_feature    1023..1862
                     /label=BBa_P0440
     misc_feature    980..1862
                     /label=BBa_S03708
     misc_feature    843..1862
                     /label=BBa_S03724
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 aattcactga gtcatatgat cgatttgggt attaaagagg agaaacaatt gatggtgagc
      121 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      181 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      241 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      301 accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac
      361 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      421 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      481 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      541 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
      601 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
      661 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      721 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      781 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag
      841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      961 ctgcgtttat atactagagt tgacagctag ctcagtccta gggactatgc tagctactag
     1021 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
     1081 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     1141 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     1201 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     1261 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     1321 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     1381 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     1441 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     1501 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     1561 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     1621 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
     1681 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca
     1741 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1801 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1861 ta
//