LOCUS       BBa_I759014             1857 bp    DNA     linear       23-Apr-2024
DEFINITION  cis2-repressed, tet-regulated YFP
ACCESSION   BBa_I759014
VERSION     BBa_I759014.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     misc_feature    55..106
                     /label=cis2 + RBS
     misc_feature    107..829
                     /label=YFP
     misc_feature    1..829
                     /label=BBa_I759034
     stem_loop       849..892
                     /label=stem_loop
     misc_feature    838..917
                     /label=BBa_B0010
     terminator      838..917
                     /label=BBa_B0010
     stem_loop       933..952
                     /label=T7 TE
     misc_feature    959..959
                     /label=stop
     polyA_site      953..966
                     /label=polya
     misc_feature    926..966
                     /label=BBa_B0012
     terminator      926..966
                     /label=BBa_B0012
     terminator      838..966
                     /label=BBa_B0015
     promoter        975..1009
                     /label=BBa_J23116
     misc_feature    1022..1025
                     /label=conserved
     RBS             1018..1029
                     /label=BBa_B0034
     CDS             1039..1655
                     /label=tetR
     misc_feature    1656..1689
                     /label=SsrA
     misc_feature    1696..1720
                     /label=Help:Barcodes
     CDS             1036..1695
                     /label=BBa_C0040
     stem_loop       1740..1783
                     /label=stem_loop
     misc_feature    1729..1808
                     /label=BBa_B0010
     terminator      1729..1808
                     /label=BBa_B0010
     stem_loop       1824..1843
                     /label=T7 TE
     misc_feature    1850..1850
                     /label=stop
     polyA_site      1844..1857
                     /label=polya
     misc_feature    1817..1857
                     /label=BBa_B0012
     terminator      1817..1857
                     /label=BBa_B0012
     misc_feature    1018..1857
                     /label=BBa_P0440
     misc_feature    975..1857
                     /label=BBa_S03708
     misc_feature    838..1857
                     /label=BBa_S03724
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 attgtttcac ctcttggatt tgggtattaa agaggagaaa caattgatgg tgagcaaggg
      121 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      181 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      241 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
      301 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt
      361 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      421 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      481 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      541 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      601 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      661 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
      721 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      781 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttatatact agagttgaca gctagctcag tcctagggac tatgctagct actagagaaa
     1021 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
     1081 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     1141 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     1201 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     1261 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     1321 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     1381 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     1441 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     1501 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     1561 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     1621 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
     1681 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa
     1741 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1801 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//