LOCUS       BBa_I759019             1861 bp    DNA     linear       25-Apr-2024
DEFINITION  cis7-repressed, tet-regulated YFP
ACCESSION   BBa_I759019
VERSION     BBa_I759019.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     misc_feature    55..110
                     /label=cis7 + RBS
     misc_feature    111..833
                     /label=YFP
     misc_feature    1..833
                     /label=BBa_I759044
     stem_loop       853..896
                     /label=stem_loop
     misc_feature    842..921
                     /label=BBa_B0010
     terminator      842..921
                     /label=BBa_B0010
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    963..963
                     /label=stop
     polyA_site      957..970
                     /label=polya
     misc_feature    930..970
                     /label=BBa_B0012
     terminator      930..970
                     /label=BBa_B0012
     terminator      842..970
                     /label=BBa_B0015
     promoter        979..1013
                     /label=BBa_J23116
     misc_feature    1026..1029
                     /label=conserved
     RBS             1022..1033
                     /label=BBa_B0034
     CDS             1043..1659
                     /label=tetR
     misc_feature    1660..1693
                     /label=SsrA
     misc_feature    1700..1724
                     /label=Help:Barcodes
     CDS             1040..1699
                     /label=BBa_C0040
     stem_loop       1744..1787
                     /label=stem_loop
     misc_feature    1733..1812
                     /label=BBa_B0010
     terminator      1733..1812
                     /label=BBa_B0010
     stem_loop       1828..1847
                     /label=T7 TE
     misc_feature    1854..1854
                     /label=stop
     polyA_site      1848..1861
                     /label=polya
     misc_feature    1821..1861
                     /label=BBa_B0012
     terminator      1821..1861
                     /label=BBa_B0012
     misc_feature    1022..1861
                     /label=BBa_P0440
     misc_feature    979..1861
                     /label=BBa_S03708
     misc_feature    842..1861
                     /label=BBa_S03724
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 aactgattca cctcttgctg tagggtaaga cagaagagga gaaacaattg atggtgagca
      121 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      181 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      241 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      301 ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact
      361 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      421 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      481 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      541 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      601 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      661 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
      721 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      781 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga
      841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      961 tgcgtttata tactagagtt gacagctagc tcagtcctag ggactatgct agctactaga
     1021 gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
     1081 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
     1141 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
     1201 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
     1261 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
     1321 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
     1381 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
     1441 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
     1501 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
     1561 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
     1621 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
     1681 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat
     1741 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1801 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1861 a
//