LOCUS       BBa_I759027             1766 bp    DNA     linear       25-Apr-2024
DEFINITION  cis3-repressed, tet-regulated Q
ACCESSION   BBa_I759027
VERSION     BBa_I759027.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     misc_feature    55..106
                     /label=cis3 + RBS
     misc_feature    107..738
                     /label=Q
     misc_feature    1..738
                     /label=BBa_I759011
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     terminator      747..875
                     /label=BBa_B0015
     promoter        884..918
                     /label=BBa_J23116
     misc_feature    931..934
                     /label=conserved
     RBS             927..938
                     /label=BBa_B0034
     CDS             948..1564
                     /label=tetR
     misc_feature    1565..1598
                     /label=SsrA
     misc_feature    1605..1629
                     /label=Help:Barcodes
     CDS             945..1604
                     /label=BBa_C0040
     stem_loop       1649..1692
                     /label=stem_loop
     misc_feature    1638..1717
                     /label=BBa_B0010
     terminator      1638..1717
                     /label=BBa_B0010
     stem_loop       1733..1752
                     /label=T7 TE
     misc_feature    1759..1759
                     /label=stop
     polyA_site      1753..1766
                     /label=polya
     misc_feature    1726..1766
                     /label=BBa_B0012
     terminator      1726..1766
                     /label=BBa_B0012
     misc_feature    927..1766
                     /label=BBa_P0440
     misc_feature    884..1766
                     /label=BBa_S03708
     misc_feature    747..1766
                     /label=BBa_S03724
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 attgattcac ctcttggagt tgggtattaa agaggagaaa caattgatga gactcgaaag
      121 cgtagctaaa tttcattcgc caaaaagccc gatgatgagc gactcaccac gggccacggc
      181 ttctgactct ctttccggta ctgatgtgat ggctgctatg gggatggcgc aatcacaagc
      241 cggattcggt atggctgcat tctgcggtaa gcacgaactc agccagaacg acaaacaaaa
      301 ggctatcaac tatctgatgc aatttgcaca caaggtatcg gggaaatacc gtggtgtggc
      361 aaagcttgaa ggaaatacta aggcaaaggt actgcaagtg ctcgcaacat tcgcttatgc
      421 ggattattgc cgtagtgccg cgacgccggg ggcaagatgc agagattgcc atggtacagg
      481 ccgtgcggtt gatattgcca aaacagagct gtgggggaga gttgtcgaga aagagtgcgg
      541 aagatgcaaa ggcgtcggct attcaaggat gccagcaagc gcagcatatc gcgctgtgac
      601 gatgctaatc ccaaacctta cccaacccac ctggtcacgc actgttaagc cgctgtatga
      661 cgctctggtg gtgcaatgcc acaaagaaga gtcaatcgca gacaacattt tgaatgcggt
      721 cacacgttag tactagagta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttgacag ctagctcagt
      901 cctagggact atgctagcta ctagagaaag aggagaaata ctagatgtcc agattagata
      961 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     1021 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     1081 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     1141 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     1201 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     1261 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     1321 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     1381 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     1441 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     1501 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     1561 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt
     1621 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1681 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1741 ttcgggtggg cctttctgcg tttata
//