BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_I759030 1 BBa_I759030 Constitutive N 2007-10-23T11:00:00Z 2015-08-31T04:08:07Z Tania Tania false false _115_ 0 253 6 Not in stock false Tania false Josh Michener component1954517 1 BBa_I759001 component1954518 1 BBa_B0010 component1954520 1 BBa_B0012 annotation1954517 1 BBa_I759001 range1954517 1 1 513 annotation1954518 1 BBa_B0010 range1954518 1 522 601 annotation1954520 1 BBa_B0012 range1954520 1 610 650 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_I759001 1 BBa_I759001 pTet-spacer-N 2007-06-26T11:00:00Z 2015-08-31T04:08:07Z Caltech iGEM Released HQ 2013 pTet-spacer-N false false _115_ 0 253 6 In stock false None true Josh Michener annotation1958469 1 spacer range1958469 1 55 112 annotation1958468 1 pTet range1958468 1 1 54 annotation1958470 1 N range1958470 1 113 513 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I759001_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacaattcactgagtcatatgatcgatttgggtattaaagaggagaaacaattgatgtgccaatcgcggggggttttcgttcaggactacaactgccacacaccaccaaagctaactgacaggagaatccagatggatgcacaaacacgccgccgcgaacgtcgcgcagagaaacaggctcaatggaaagcagcaaatcccctgttggttggggtaagcgcaaaaccagttaaccgccctattctctcgctgaatcgcaaaccgaaatcacgagtagaaagcgcactaaatccgatagaccttacagtgctggctgaataccacaaacagattgaaagcaacctgcaacgtattgagcgcaagaatcagcgcacatggtacagcaagcctggcgaacgcggcataacatgcagtggacgccagaaaattaagggaaaatcgattcctcttatctag BBa_I759030_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacaattcactgagtcatatgatcgatttgggtattaaagaggagaaacaattgatgtgccaatcgcggggggttttcgttcaggactacaactgccacacaccaccaaagctaactgacaggagaatccagatggatgcacaaacacgccgccgcgaacgtcgcgcagagaaacaggctcaatggaaagcagcaaatcccctgttggttggggtaagcgcaaaaccagttaaccgccctattctctcgctgaatcgcaaaccgaaatcacgagtagaaagcgcactaaatccgatagaccttacagtgctggctgaataccacaaacagattgaaagcaacctgcaacgtattgagcgcaagaatcagcgcacatggtacagcaagcctggcgaacgcggcataacatgcagtggacgccagaaaattaagggaaaatcgattcctcttatctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z