LOCUS       BBa_I760010             1737 bp    DNA     linear       09-May-2024
DEFINITION  Trigger-1
ACCESSION   BBa_I760010
VERSION     BBa_I760010.1  
FEATURES             Location/Qualifiers
     promoter        1..16
                     /label=BBa_I760005
     misc_feature    25..67
                     /label=OR2
     misc_feature    32..45
                     /label=OR2
     misc_feature    54..67
                     /label=OR1
     promoter        25..30
                     /label=-35
     promoter        48..53
                     /label=-10
     promoter        26..31
                     /label=-35
     misc_feature    59..61
                     /label=start
     misc_feature    25..70
                     /label=BBa_R0052
     promoter        25..70
                     /label=BBa_R0052
     misc_feature    83..86
                     /label=conserved
     RBS             79..90
                     /label=BBa_B0034
     CDS             100..807
                     /label=cI lambda
     misc_feature    808..840
                     /label=LVA
     misc_feature    847..871
                     /label=Help:Barcodes
     CDS             97..846
                     /label=BBa_C0051
     misc_feature    79..871
                     /label=BBa_J06550
     CDS             878..1585
                     /label=mCherry
     misc_feature    1229..1229
                     /label=C->T (removing PstI site)
     misc_feature    1586..1594
                     /label=LVA
     misc_feature    878..1600
                     /label=BBa_J06505
     misc_feature    1609..1649
                     /label=B0012
     misc_feature    1658..1737
                     /label=B0010
     terminator      1609..1737
                     /label=BBa_B0025
ORIGIN
        1 atgacaaaat tgtcattact agagttgaca aacaagatac attgtatgaa aatacaagaa
       61 agtttgttga tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta
      121 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat
      181 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt
      241 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa
      301 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg
      361 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct
      421 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag
      481 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt
      541 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc
      601 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat
      661 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac
      721 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct
      781 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct
      841 taataacgct gatagtgcta gtgtagatcg ctactagatg gtgagcaagg gcgaggagga
      901 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      961 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1021 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1081 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1141 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1201 cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa
     1261 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1321 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1381 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1441 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     1501 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1561 cggcggcatg gacgagctgt acaagttagt agcttaataa tactagagta taaacgcaga
     1621 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac
     1681 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctgg
//