LOCUS       BBa_I760011             1840 bp    DNA     linear       13-May-2024
DEFINITION  Trigger-1
ACCESSION   BBa_I760011
VERSION     BBa_I760011.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     promoter        246..251
                     /label=-10
     promoter        223..228
                     /label=-35
     misc_feature    233..249
                     /label=OR1
     misc_feature    209..225
                     /label=OR2
     misc_feature    209..257
                     /label=BBa_R0051
     promoter        209..257
                     /label=BBa_R0051
     misc_feature    270..273
                     /label=conserved
     RBS             266..277
                     /label=BBa_B0034
     CDS             284..952
                     /label=cI 434
     misc_feature    914..952
                     /label=LVA
     misc_feature    284..952
                     /label=BBa_C0052
     misc_feature    953..977
                     /label=Help:Barcodes
     CDS             284..952
                     /label=BBa_C0052
     CDS             984..1703
                     /label=GFP protein
     CDS             984..1703
                     /label=BBa_E0040
     misc_feature    1712..1752
                     /label=B0012
     misc_feature    1761..1840
                     /label=B0010
     terminator      1712..1840
                     /label=BBa_B0025
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagta acaccgtgcg tgttgactat tttacctctg
      241 gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatgagta tttcttccag
      301 ggtaaaaagc aaaagaatcc agcttggact taaccaggct gaacttgctc aaaaggtggg
      361 gactacccag cagtctatag agcagctcga aaacggtaaa actaagcgac cacgcttttt
      421 accagaactt gcgtcagctc ttggcgtaag tgttgactgg ctgctcaatg gcacctctga
      481 ttcgaatgtt agatttgttg ggcacgttga gcccaaaggg aaatatccat tgattagcat
      541 ggttagagct ggttcgtggt gtgaagcttg tgaaccctac gatatcaagg acattgatga
      601 atggtatgac agtgacgtta acttattagg caatggattc tggctgaagg ttgaaggtga
      661 ttccatgacc tcacctgtag gtcaaagcat ccctgaaggt catatggtgt tagtagatac
      721 tggacgggag ccagtgaatg gaagccttgt tgtagccaaa ctgactgacg cgaacgaagc
      781 aacattcaag aaactggtca tagatggcgg tcagaagtac ctgaaaggcc tgaatccttc
      841 atggcctatg actcctatca acggaaactg caagattatc ggtgttgtcg tggaagcgag
      901 ggtaaaattc gtagctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata
      961 gtgctagtgt agatctctac tagatgcgta aaggagaaga acttttcact ggagttgtcc
     1021 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     1081 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     1141 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     1201 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     1261 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     1321 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     1381 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     1441 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     1501 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     1561 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     1621 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1681 tggatgaact atacaaataa taatactaga gtataaacgc agaaaggccc acccgaaggt
     1741 gagccagtgt gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc
     1801 cagtctttcg actgagcctt tcgttttatt tgatgcctgg
//