LOCUS BBa_I761009 4188 bp DNA linear 17-May-2024 DEFINITION Complete system: GlucOperon ACCESSION BBa_I761009 VERSION BBa_I761009.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 misc_feature 138..222 /label=TAT peptide export signal CDS 223..285 /label=Human insulin A fragment CDS 135..285 /label=BBa_I761002 misc_feature 298..301 /label=conserved RBS 294..305 /label=BBa_B0034 misc_feature 315..399 /label=TAT peptide export signal CDS 400..491 /label=Human insulin B chain CDS 312..497 /label=BBa_I761003 stem_loop 513..532 /label=T7 TE misc_feature 539..539 /label=stop polyA_site 533..546 /label=polya misc_feature 506..546 /label=BBa_B0012 terminator 506..546 /label=BBa_B0012 stem_loop 567..589 /label=stem_loop misc_feature 555..600 /label=BBa_B0011 terminator 555..600 /label=BBa_B0011 misc_feature 609..828 /label=unidentified/uncharacterized CinR dependent stem_loop 643..682 /label=stem_loop stem_loop 699..744 /label=stem_loop promoter 609..833 /label=BBa_R0078 misc_feature 843..860 /label=C1 OmpR misc_feature 863..881 /label=C2 OmpR misc_feature 884..901 /label=C3 OmpR promoter 842..903 /label=BBa_I761001 misc_feature 916..919 /label=conserved RBS 912..923 /label=BBa_B0034 misc_feature 933..1017 /label=TAT peptide export signal CDS 1018..4079 /label=Mouse insulin degradation enzyme CDS 930..4085 /label=BBa_I761004 stem_loop 4101..4120 /label=T7 TE misc_feature 4127..4127 /label=stop polyA_site 4121..4134 /label=polya misc_feature 4094..4134 /label=BBa_B0012 terminator 4094..4134 /label=BBa_B0012 stem_loop 4155..4177 /label=stem_loop misc_feature 4143..4188 /label=BBa_B0011 terminator 4143..4188 /label=BBa_B0011 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatggac aaattcgacg ctaatcgccg caaattgctg gcgcttggtg 181 gcgttgcact cggtgccgcc atcctgccga cccctgcgtt tgcaggcatt gtggaacaat 241 gctgtaccag catctgctcc ctctaccagc tggagaacta ctgcatacta gagaaagagg 301 agaaatacta gatggacaaa ttcgacgcta atcgccgcaa attgctggcg cttggtggcg 361 ttgcactcgg tgccgccatc ctgccgaccc ctgcgtttgc atttgtgaac caacacctgt 421 gcggctcaca cctggtggaa gctctctacc tagtgtgcgg ggaacgaggc ttcttctaca 481 cacccaagac ctaataatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 541 tttatatact agagagagaa tataaaaagc cagattatta atccggcttt tttattattt 601 tactagagcc ctttgtgcgt ccaaacggac gcacggcgct ctaaagcggg tcgcgatctt 661 tcagattcgc tcctcgcgct ttcagtcttt gttttggcgc atgtcgttat cgcaaaaccg 721 ctgcacactt ttgcgcgaca tgctctgatc cccctcatct gggggggcct atctgaggga 781 atttccgatc cggctcgcct gaaccattct gctttccacg aacttgaaaa cgctactaga 841 gtacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta gtatcatatt 901 cgttactaga gaaagaggag aaatactaga tggacaaatt cgacgctaat cgccgcaaat 961 tgctggcgct tggtggcgtt gcactcggtg ccgccatcct gccgacccct gcgtttgcaa 1021 tgcggaacgg gctcgtgtgg ctgctgcacc ccgcgctgcc cggcaccttg cgctccatcc 1081 tcggcgcccg cccgccgccc gcgaagcgac tgtgtggatt cccaaaacag acttacagca 1141 caatgagtaa tccggccatc cagagaatag aagaccaaat tgtcaagtct cctgaagaca 1201 aacgggaata ccgtggacta gagctggcca atggcatcaa agtgcttctc atcagcgatc 1261 ccaccacaga caagtcctca gcggccctcg atgtgcacat aggttcactg tcagaccctc 1321 caaatattcc tggcttaagt catttttgtg aacatatgct gtttttggga accaagaaat 1381 atcctaaaga aaatgaatat agccagtttc tcagtgaaca tgctggaagt tcaaatgcat 1441 tcaccagtgg agaacacacc aattattatt tcgatgtttc ccatgaacac ttggaaggag 1501 ccctggacag gtttgcgcag tttttcctgt gccccttgtt tgatgcaagt tgtaaagaca 1561 gagaggtgaa cgctgtcgat tcagaacatg agaagaatgt gatgaacgat gcctggagac 1621 tcttccagct ggaaaaggct acggggaacc ccaaacaccc cttcagcaaa tttgggacag 1681 gaaacaaata tactctagag actcggccca accaagaagg catcgacgta agggaagagc 1741 tcttgaaatt tcactctacg tattattcgt ccaatctgat ggcgatttgt gttttaggtc 1801 gagaatcctt agacgacctc actaacctgg tggtgaagct gttctccgag gtggagaaca 1861 agaacgttcc tctgcctgag ttccctgagc accctttcca ggaagagcat ctcaggcaac 1921 tttataaaat agtacccatt aaggatatta gaaatcttta tgtgacattt cccataccag 1981 accttcagca gtactacaaa tccaatcctg gtcattatct tggtcatcta attgggcacg 2041 aaggtcctgg aagcctgttg tcagaactca aatcaaaggg ctgggtgaat accctggttg 2101 gtggacagaa ggaaggagcc cgaggtttta tgttttttat cattaatgtg gacttaactg 2161 aagaagggtt attgcacgtt gaagatataa ttttgcacat gtttcaatac attcagaagc 2221 tacgtgcaga aggacctcaa gaatgggttt tccaagagtg caaggacttg aatgctgttg 2281 ctttcagatt taaagataaa gagagaccac gaggctatac gtccaagatt gcaggaaagt 2341 tgcactatta tcccctaaat ggagtgctca cagctgaata tctattggag gaatttagac 2401 ccgacttgat agacatggtt cttgataaac tcagaccaga aaatgtgcgg gtggcaatag 2461 tttccaaatc atttgaaggg aaaactgacc gcacagagca gtggtatgga acacagtata 2521 agcaagaagc tatcccagag gacatcattc agaaatggca aaatgctgac cttaatggaa 2581 aatttaagct tccaacaaag aatgaattta ttcccacaaa ttttgagatt ttgtcattag 2641 aaaaagatgc aacaccatac cctgctctta ttaaggatac agccatgagt aagctgtggt 2701 tcaaacaaga tgataaattt ttcttgccga aagcttgtct caactttgaa tttttcagcc 2761 cgtttgctta tgtggacccc ttgcactgta acatggccta tttgtacctt gagctcctca 2821 aagactcact caacgagtat gcatatgcag cagagctagc aggcctgagc tatgacctcc 2881 aaaataccat ctatgggatg tatctctcag tgaaaggtta caatgacaaa cagccaattt 2941 tgctgaagaa gatcaccgag aaaatggcta cttttgagat tgataagaag agatttgaaa 3001 ttatcaaaga ggcctatatg cgatctctta ataacttccg ggctgagcag cctcaccagc 3061 atgccatgta ctacctccgc ttgctgatga ctgaagtggc ctggactaaa gatgagctaa 3121 aagaagccct tgacgatgtt accctccccc gacttaaggc cttcatacct cagctgctgt 3181 cacggctgca tattgaagca cttctccatg gaaacataac caagcaggct gccttaggag 3241 ttatgcagat ggtagaagac actcttatcg agcatgctca caccaaacct ctccttccaa 3301 gtcagctagt ccggtacaga gaagtccagc tccctgacag aggatggttt gtttatcagc 3361 agaggaatga agttcacaat aactgcggca tcgagatcta ctaccagaca gacatgcaga 3421 gcacctcgga gaacatgttc ctggagctgt tttgccagat tatctcggag ccttgcttca 3481 ataccctgcg caccaaggag cagcttggct atattgtctt cagtggaccc cgtcgggcca 3541 acggtatcca aggcttgcgc ttcatcatcc agtcagaaaa accaccccac tacctggaga 3601 gcagagtaga agccttcttg attaccatgg aaaaggccat agaggacatg acagaggagg 3661 cgttccaaaa acacattcaa gcactagcga ttcggcgact cgacaaacca aagaaactct 3721 ctgcggagtg tgctaaatac tggggggaaa tcatctcgca gcagtacaat tacgacagag 3781 ataacataga agttgcatat ttaaagacac ttaccaagga cgatatcatc agattctacc 3841 aggaaatgtt ggctgtcgac gcaccgagga gacataaagt gtctgtccat gttcttgcca 3901 gggaaatgga ttcttgtcct gttgttggag agttcccctc tcagaatgat ataaacttgt 3961 ccgaagcgcc acccttgcca caacctgaag tgattcacaa catgactgag ttcaagcgtg 4021 gcctgccact gtttcccctc gtgaaaccac atattaactt catggctgca aaactctgat 4081 aataatacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 4141 agagagaata taaaaagcca gattattaat ccggcttttt tattattt //