LOCUS       BBa_I763006             1903 bp    DNA     linear       17-May-2024
DEFINITION  cI (+LVA) repressor regulated by pLac Promoter, GFP (+LVA)
ACCESSION   BBa_I763006
VERSION     BBa_I763006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..937
                     /label=cI lambda
     misc_feature    938..970
                     /label=LVA
     misc_feature    977..1001
                     /label=Help:Barcodes
     CDS             227..976
                     /label=BBa_C0051
     misc_feature    1008..1727
                     /label=BBa_E0040
     misc_feature    1722..1760
                     /label=LVA
     misc_feature    1761..1766
                     /label=Double Stop
     misc_feature    1008..1010
                     /label=Start
     misc_feature    1008..1766
                     /label=BBa_J04031
     stem_loop       1786..1829
                     /label=stem_loop
     misc_feature    1775..1854
                     /label=BBa_B0010
     terminator      1775..1854
                     /label=BBa_B0010
     stem_loop       1870..1889
                     /label=T7 TE
     misc_feature    1896..1896
                     /label=stop
     polyA_site      1890..1903
                     /label=polya
     misc_feature    1863..1903
                     /label=BBa_B0012
     terminator      1863..1903
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa
      241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc
      961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagatg cgtaaaggag
     1021 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     1081 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     1141 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
     1201 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
     1261 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     1321 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     1381 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     1441 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     1501 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1561 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1621 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1681 cagctgctgg gattacacat ggcatggatg aactatacaa aaggcctgct gcaaacgacg
     1741 aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa acgaaaggct
     1801 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1861 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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