LOCUS       BBa_I763037             2228 bp    DNA     linear       17-May-2024
DEFINITION  RBS-LacY-RBS-GFP-T
ACCESSION   BBa_I763037
VERSION     BBa_I763037.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1306
                     /label=BBa_J22101
     misc_feature    1319..1322
                     /label=conserved
     RBS             1315..1326
                     /label=BBa_B0034
     misc_feature    1333..2052
                     /label=BBa_E0040
     misc_feature    2047..2085
                     /label=LVA
     misc_feature    2086..2091
                     /label=Double Stop
     misc_feature    1333..1335
                     /label=Start
     misc_feature    1333..2091
                     /label=BBa_J04031
     stem_loop       2111..2154
                     /label=stem_loop
     misc_feature    2100..2179
                     /label=BBa_B0010
     terminator      2100..2179
                     /label=BBa_B0010
     stem_loop       2195..2214
                     /label=T7 TE
     misc_feature    2221..2221
                     /label=stop
     polyA_site      2215..2228
                     /label=polya
     misc_feature    2188..2228
                     /label=BBa_B0012
     terminator      2188..2228
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta
       61 tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact tttttatcat
      121 gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata tcagcaaaag
      181 tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc aaccgctgtt
      241 tggtctgctt tctgacaaac tcgggctgcg caaatacctg ctgtggatta ttaccggcat
      301 gttagtgatg tttgcgccgt tctttatttt tatcttcggg ccactgttac aatacaacat
      361 tttagtagga tcgattgttg gtggtattta tctaggcttt tgttttaacg ccggtgcgcc
      421 agcagtagag gcatttattg agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc
      481 gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca tcatgttcac
      541 catcaataat cagtttgttt tctggctggg ctctggctgt gcactcatcc tcgccgtttt
      601 actctttttc gccaaaacgg atgcgccctc ttctgccacg gttgccaatg cggtaggtgc
      661 caaccattcg gcatttagcc ttaagctggc actggaactg ttcagacagc caaaactgtg
      721 gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg accaacagtt
      781 tgctaatttc tttacttcgt tctttgctac cggtgaacag ggtacgcggg tatttggcta
      841 cgtaacgaca atgggcgaat tacttaacgc ctcgattatg ttctttgcgc cactgatcat
      901 taatcgcatc ggtgggaaaa acgccctgct gctggctggc actattatgt ctgtacgtat
      961 tattggctca tcgttcgcca cctcagcgct ggaagtggtt attctgaaaa cgctgcatat
     1021 gtttgaagta ccgttcctgc tggtgggctg ctttaaatat attaccagcc agtttgaagt
     1081 gcgtttttca gcgacgattt atctggtctg tttctgcttc tttaagcaac tggcgatgat
     1141 ttttatgtct gtactggcgg gcaatatgta tgaaagcatc ggtttccagg gcgcttatct
     1201 ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc ttagcggccc
     1261 cggcccgctt tccctgctgc gtcgtcaggt gaatgaagtc gcttaatact agagaaagag
     1321 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1381 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1441 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1501 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1561 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1621 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1681 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1741 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1801 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1861 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1921 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1981 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     2041 tacaaaaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc
     2101 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2161 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2221 cgtttata
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