LOCUS BBa_I763040 2053 bp DNA linear 17-May-2024 DEFINITION RFP regulated by TetR and GFP regulated by pLac ACCESSION BBa_I763040 VERSION BBa_I763040.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..755 /label=mrfp1 misc_feature 762..786 /label=Help:Barcodes CDS 81..761 /label=BBa_E1010 stem_loop 806..849 /label=stem_loop misc_feature 795..874 /label=BBa_B0010 terminator 795..874 /label=BBa_B0010 stem_loop 890..909 /label=T7 TE misc_feature 916..916 /label=stop polyA_site 910..923 /label=polya misc_feature 883..923 /label=BBa_B0012 terminator 883..923 /label=BBa_B0012 CDS 932..1019 /label=end of LacI coding region (inactive) misc_feature 932..1131 /label=BBa_R0010 misc_feature 1020..1057 /label=CAP binding site promoter 1068..1073 /label=-35 promoter 1092..1097 /label=-10 misc_feature 1097..1131 /label=LacI binding site misc_feature 1104..1104 /label=start promoter 932..1131 /label=BBa_R0010 misc_feature 1144..1147 /label=conserved RBS 1140..1151 /label=BBa_B0034 misc_feature 1158..1877 /label=BBa_E0040 misc_feature 1872..1910 /label=LVA misc_feature 1911..1916 /label=Double Stop misc_feature 1158..1160 /label=Start misc_feature 1158..1916 /label=BBa_J04031 stem_loop 1936..1979 /label=stem_loop misc_feature 1925..2004 /label=BBa_B0010 terminator 1925..2004 /label=BBa_B0010 stem_loop 2020..2039 /label=T7 TE misc_feature 2046..2046 /label=stop polyA_site 2040..2053 /label=polya misc_feature 2013..2053 /label=BBa_B0012 terminator 2013..2053 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt 121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg 181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc 241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta 301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg 361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc 421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc 481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag 541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg 601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca 661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt 721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta 781 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 901 gggtgggcct ttctgcgttt atatactaga gcaatacgca aaccgcctct ccccgcgcgt 961 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 1021 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 1081 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga 1141 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 1201 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 1261 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 1321 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 1381 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 1441 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 1501 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 1561 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 1621 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 1681 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 1741 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 1801 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 1861 aactatacaa aaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact 1921 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1981 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2041 ttctgcgttt ata // LOCUS BBa_I763034 2053 bp DNA linear 17-May-2024 DEFINITION PTetR and PLac activities tester ACCESSION BBa_I763034 VERSION BBa_I763034.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..755 /label=mrfp1 misc_feature 762..786 /label=Help:Barcodes CDS 81..761 /label=BBa_E1010 stem_loop 806..849 /label=stem_loop misc_feature 795..874 /label=BBa_B0010 terminator 795..874 /label=BBa_B0010 stem_loop 890..909 /label=T7 TE misc_feature 916..916 /label=stop polyA_site 910..923 /label=polya misc_feature 883..923 /label=BBa_B0012 terminator 883..923 /label=BBa_B0012 misc_feature 63..923 /label=BBa_I13507 misc_feature 1..923 /label=BBa_I763033 CDS 932..1019 /label=end of LacI coding region (inactive) misc_feature 932..1131 /label=BBa_R0010 misc_feature 1020..1057 /label=CAP binding site promoter 1068..1073 /label=-35 promoter 1092..1097 /label=-10 misc_feature 1097..1131 /label=LacI binding site misc_feature 1104..1104 /label=start promoter 932..1131 /label=BBa_R0010 misc_feature 1144..1147 /label=conserved RBS 1140..1151 /label=BBa_B0034 misc_feature 1158..1877 /label=BBa_E0040 misc_feature 1872..1910 /label=LVA misc_feature 1911..1916 /label=Double Stop misc_feature 1158..1160 /label=Start misc_feature 1158..1916 /label=BBa_J04031 stem_loop 1936..1979 /label=stem_loop misc_feature 1925..2004 /label=BBa_B0010 terminator 1925..2004 /label=BBa_B0010 stem_loop 2020..2039 /label=T7 TE misc_feature 2046..2046 /label=stop polyA_site 2040..2053 /label=polya misc_feature 2013..2053 /label=BBa_B0012 terminator 2013..2053 /label=BBa_B0012 misc_feature 932..2053 /label=BBa_I763004 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt 121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg 181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc 241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta 301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg 361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc 421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc 481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag 541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg 601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca 661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt 721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta 781 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 901 gggtgggcct ttctgcgttt atatactaga gcaatacgca aaccgcctct ccccgcgcgt 961 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 1021 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 1081 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga 1141 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 1201 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 1261 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 1321 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 1381 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 1441 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 1501 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 1561 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 1621 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 1681 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 1741 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 1801 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 1861 aactatacaa aaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact 1921 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1981 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2041 ttctgcgttt ata //