LOCUS       BBa_I764000             1000 bp    DNA     linear       23-May-2024
DEFINITION  production of tetR under the arabinose regulated promoter (AraC)
ACCESSION   BBa_I764000
VERSION     BBa_I764000.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     RBS             158..172
                     /label=RBS-1\Strong
     RBS             165..169
                     /label=RBS
     misc_feature    158..172
                     /label=BBa_B0030
     RBS             158..172
                     /label=BBa_B0030
     CDS             182..798
                     /label=tetR
     misc_feature    799..832
                     /label=SsrA
     misc_feature    839..863
                     /label=Help:Barcodes
     CDS             179..863
                     /label=BBa_C0040
     stem_loop       883..926
                     /label=stem_loop
     misc_feature    872..951
                     /label=BBa_B0010
     terminator      872..951
                     /label=BBa_B0010
     stem_loop       967..986
                     /label=T7 TE
     misc_feature    993..993
                     /label=stop
     polyA_site      987..1000
                     /label=polya
     misc_feature    960..1000
                     /label=BBa_B0012
     terminator      960..1000
                     /label=BBa_B0012
     terminator      872..1000
                     /label=BBa_B0015
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagatt aaagaggaga aatactagat
      181 gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg
      241 aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt
      301 gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga
      361 taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa
      421 taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt
      481 aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg
      541 ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac
      601 tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc
      661 tactactgat agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg
      721 tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact
      781 taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca
      841 ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata
//