LOCUS BBa_I8020 5891 bp DNA linear 08-May-2024 DEFINITION Booperator test: LuxR biasing, LuxR/RhlR crosstalk ACCESSION BBa_I8020 VERSION BBa_I8020.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 820..825 /label=2 CDS 82..825 /label=cI HK022 misc_feature 787..819 /label=SsrA misc_feature 82..89 /label=OR3 partial misc_feature 82..825 /label=BBa_C0050 misc_feature 826..850 /label=Help:Barcodes CDS 82..825 /label=BBa_C0050 stem_loop 870..913 /label=stem_loop misc_feature 859..938 /label=BBa_B0010 terminator 859..938 /label=BBa_B0010 stem_loop 954..973 /label=T7 TE misc_feature 980..980 /label=stop polyA_site 974..987 /label=polya misc_feature 947..987 /label=BBa_B0012 terminator 947..987 /label=BBa_B0012 promoter 1003..1008 /label=-35 RBS 1008..1022 /label=OR2 promoter 1025..1030 /label=-10 RBS 1032..1046 /label=OR1 misc_feature 1038..1038 /label=putative misc_feature 996..1050 /label=BBa_R0050 promoter 996..1050 /label=BBa_R0050 misc_feature 1063..1066 /label=conserved RBS 1059..1070 /label=BBa_B0034 CDS 1077..1838 /label=YFP (LVA) misc_feature 1833..1838 /label=2 misc_feature 1794..1832 /label=SsrA misc_feature 1318..1318 /label=A (Q->L) misc_feature 1077..1838 /label=BBa_E0032 misc_feature 1077..1838 /label=BBa_E0032 stem_loop 1858..1901 /label=stem_loop misc_feature 1847..1926 /label=BBa_B0010 terminator 1847..1926 /label=BBa_B0010 stem_loop 1942..1961 /label=T7 TE misc_feature 1968..1968 /label=stop polyA_site 1962..1975 /label=polya misc_feature 1935..1975 /label=BBa_B0012 terminator 1935..1975 /label=BBa_B0012 misc_feature 1984..2003 /label=LuxR/HSL promoter 2003..2008 /label=-35 promoter 2025..2030 /label=-10 misc_feature 2036..2036 /label=start misc_feature 1984..2038 /label=BBa_R0062 promoter 1984..2038 /label=BBa_R0062 misc_feature 2051..2054 /label=conserved RBS 2047..2058 /label=BBa_B0034 CDS 2065..2826 /label=CFP (LVA) misc_feature 2783..2820 /label=SsrA misc_feature 2821..2826 /label=2 misc_feature 2133..2133 /label=A misc_feature 2065..2826 /label=BBa_E0022 misc_feature 2065..2826 /label=BBa_E0022 stem_loop 2846..2889 /label=stem_loop misc_feature 2835..2914 /label=BBa_B0010 terminator 2835..2914 /label=BBa_B0010 stem_loop 2930..2949 /label=T7 TE misc_feature 2956..2956 /label=stop polyA_site 2950..2963 /label=polya misc_feature 2923..2963 /label=BBa_B0012 terminator 2923..2963 /label=BBa_B0012 misc_feature 2972..2990 /label=TetR 1 misc_feature 2972..3025 /label=BBa_R0040 promoter 2991..2996 /label=-35 misc_feature 2997..3015 /label=TetR 2 promoter 3014..3019 /label=-10 promoter 2972..3025 /label=BBa_R0040 misc_feature 3038..3041 /label=conserved RBS 3034..3045 /label=BBa_B0034 CDS 3055..3671 /label=tetR misc_feature 3672..3705 /label=SsrA misc_feature 3712..3736 /label=Help:Barcodes CDS 3052..3711 /label=BBa_C0040 stem_loop 3756..3799 /label=stem_loop misc_feature 3745..3824 /label=BBa_B0010 terminator 3745..3824 /label=BBa_B0010 stem_loop 3840..3859 /label=T7 TE misc_feature 3866..3866 /label=stop polyA_site 3860..3873 /label=polya misc_feature 3833..3873 /label=BBa_B0012 terminator 3833..3873 /label=BBa_B0012 misc_feature 3882..3900 /label=TetR 1 misc_feature 3882..3935 /label=BBa_R0040 promoter 3901..3906 /label=-35 misc_feature 3907..3925 /label=TetR 2 promoter 3924..3929 /label=-10 promoter 3882..3935 /label=BBa_R0040 misc_feature 3948..3951 /label=conserved RBS 3944..3955 /label=BBa_B0034 CDS 3962..4684 /label=rhlR misc_feature 4685..4717 /label=LVA misc_feature 4724..4748 /label=Help:Barcodes CDS 3962..4723 /label=BBa_C0071 stem_loop 4768..4811 /label=stem_loop misc_feature 4757..4836 /label=BBa_B0010 terminator 4757..4836 /label=BBa_B0010 stem_loop 4852..4871 /label=T7 TE misc_feature 4878..4878 /label=stop polyA_site 4872..4885 /label=polya misc_feature 4845..4885 /label=BBa_B0012 terminator 4845..4885 /label=BBa_B0012 misc_feature 4894..4912 /label=TetR 1 misc_feature 4894..4947 /label=BBa_R0040 promoter 4913..4918 /label=-35 misc_feature 4919..4937 /label=TetR 2 promoter 4936..4941 /label=-10 promoter 4894..4947 /label=BBa_R0040 misc_feature 4960..4963 /label=conserved RBS 4956..4967 /label=BBa_B0034 misc_feature 4974..4975 /label=prefix misc_feature 5147..5147 /label=T misc_feature 5465..5465 /label=A CDS 4974..5723 /label=luxR misc_feature 4974..5729 /label=BBa_C0062 misc_feature 5730..5754 /label=Help:Barcodes CDS 4974..5729 /label=BBa_C0062 stem_loop 5774..5817 /label=stem_loop misc_feature 5763..5842 /label=BBa_B0010 terminator 5763..5842 /label=BBa_B0010 stem_loop 5858..5877 /label=T7 TE misc_feature 5884..5884 /label=stop polyA_site 5878..5891 /label=polya misc_feature 5851..5891 /label=BBa_B0012 terminator 5851..5891 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggttcaa cagaaagagc gtgaaacttt ctcgcagagg 121 cttgcgctgg cctgtgataa agcgggatta cctttgcatg gtaggcaggc tgatttagct 181 gtcaggctta aggtcacacc aaaagccatt agtaaatggt tcaacgggga gtcaatacca 241 agaaaagaca agatggaatc tctggcttcg gtgctgggaa ctactgctgc atatctgcat 301 ggctatgctg atgatgacgg tatcacggta aatcatctat caagatcaaa tgattattat 361 cgtgttgatg tattggatgt tcaggcgagc gccgggccag gaaccatggt ttccaatgaa 421 tttatagaaa agataagagc aattgaatat acgaccgagc aggcaagaat tttatttaat 481 ggaaggccac aggaaagcgt aaaagtcatc acggttcgcg gtgacagcat ggagggaacc 541 atcaatccgg gagatgagat ctttgttgat gtatccataa cctgttttga tggcgatggc 601 atttatgtgt ttgtatacgg gaaaacaatg cacgttaagc gcctgcaaat gcaaaagaac 661 aggcttgccg tcatctctga caatgccgct tatgatcgat ggtacataga agaaggtgaa 721 gaagagcaac ttcacattct agccaaagtc ctcattaggc agtcaatcga ttacaagcga 781 ttcggagctg caaacgacga aaactacgct ttagtagctt aataaccctg atagtgctag 841 tgtagatccc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 961 cttcgggtgg gcctttctgc gtttatatac tagagttagg tattgactgt actatcagtt 1021 ccgtcataat atgaaccata agttcaccac tactagagaa agaggagaaa tactagatgg 1081 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 1141 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 1201 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 1261 tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc 1321 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 1381 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 1441 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 1501 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 1561 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 1621 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 1681 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 1741 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg 1801 ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata 1861 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1921 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 1981 gagacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaataaata 2041 ctagagaaag aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg 2101 gtgcccatcc tggtcgagct ggacggcgac gtgaacggcc acaagttcag cgtgtccggc 2161 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 2221 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc 2281 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 2341 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 2401 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 2461 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat 2521 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc 2581 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 2641 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 2701 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 2761 ggcatggacg agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct 2821 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 2881 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 2941 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc 3001 tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta gatgtccaga 3061 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa 3121 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg 3181 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac 3241 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct 3301 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca 3361 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa 3421 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt 3481 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact 3541 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag 3601 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt 3661 gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag 3721 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 3781 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 3841 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga 3901 ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta 3961 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc 4021 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt 4081 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt 4141 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt 4201 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct 4261 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc 4321 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca 4381 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga 4441 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg 4501 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga 4561 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca 4621 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct 4681 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg 4741 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 4801 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 4861 tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga gattgacatc 4921 cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac tagatgaaaa 4981 acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata 5041 atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac 5101 tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc 5161 ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag 5221 attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa 5281 ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta 5341 gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa 5401 aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc 5461 cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt 5521 taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata 5581 tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa 5641 tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa 5701 ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag 5761 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 5821 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 5881 ctgcgtttat a //