LOCUS       BBa_I8540               2525 bp    DNA     linear       11-May-2024
DEFINITION  PoPS->Inverter->GFP
ACCESSION   BBa_I8540
VERSION     BBa_I8540.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..1449
                     /label=BBa_C2006
     stem_loop       1469..1512
                     /label=stem_loop
     misc_feature    1458..1537
                     /label=BBa_B0010
     terminator      1458..1537
                     /label=BBa_B0010
     stem_loop       1553..1572
                     /label=T7 TE
     misc_feature    1579..1579
                     /label=stop
     polyA_site      1573..1586
                     /label=polya
     misc_feature    1546..1586
                     /label=BBa_B0012
     terminator      1546..1586
                     /label=BBa_B0012
     promoter        1604..1609
                     /label=-35
     promoter        1627..1632
                     /label=-10
     misc_feature    1614..1627
                     /label=Zif23
     misc_feature    1595..1603
                     /label=consensus
     misc_feature    1639..1639
                     /label=(+1)
     promoter        1595..1639
                     /label=BBa_R2000
     RBS             1648..1662
                     /label=RBS-1\Strong
     RBS             1655..1659
                     /label=RBS
     misc_feature    1648..1662
                     /label=BBa_B0030
     RBS             1648..1662
                     /label=BBa_B0030
     CDS             1669..2388
                     /label=GFP protein
     CDS             1669..2388
                     /label=BBa_E0040
     stem_loop       2408..2451
                     /label=stem_loop
     misc_feature    2397..2476
                     /label=BBa_B0010
     terminator      2397..2476
                     /label=BBa_B0010
     stem_loop       2492..2511
                     /label=T7 TE
     misc_feature    2518..2518
                     /label=stop
     polyA_site      2512..2525
                     /label=polya
     misc_feature    2485..2525
                     /label=BBa_B0012
     terminator      2485..2525
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatgaaaatc gaagaaggta aactggtaat ctggattaac
       61 ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga
      121 attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca
      181 actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa
      241 tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt
      301 acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgttgaagcg
      361 ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc
      421 ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa
      481 gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt caagtatgaa
      541 aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa agcgggtctg
      601 accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga ttactccatc
      661 gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg
      721 tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac cttcaagggt
      781 caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac
      841 aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg tctggaagcg
      901 gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga agagttggcg
      961 aaagatccac gtattgccgc cactatggaa aacgcccaga aaggtgaaat catgccgaac
     1021 atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc
     1081 ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctaattcgag ctcgaacaac
     1141 aacaacaata acaataacaa caacctcggg atcgagggaa ggatgaaacc gttccagtgc
     1201 cgtatctgca tgcgtaactt ctcccgttcc gaccacctga ccacccacat ccgtacccac
     1261 accggtgaaa aaccgttcgc ttgcgacatc tgcggtcgta aattcgctcg ttccgacgaa
     1321 cgtaaacgtc accgtaaatt gcagcacatg aaacagctgg aagacaaagt tgaagaactg
     1381 ctgtccaaaa actaccacct ggaaaacgaa gttgctcgtc tgaaaaaact ggttggtgaa
     1441 cgttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1501 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1561 ttcgggtggg cctttctgcg tttatatact agagagttta ttcttgacat ggtcccacgc
     1621 gcgtgggata ctacgtcagt actagagatt aaagaggaga aatactagat gcgtaaagga
     1681 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1741 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1801 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     1861 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     1921 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1981 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     2041 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     2101 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     2161 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     2221 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     2281 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     2341 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
     2401 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2461 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2521 ttata
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