LOCUS       BBa_J01073              1291 bp    DNA     linear       18-May-2024
DEFINITION  TEST [OriTF][r0051][StrRBS][RFP][DblTerminator]
ACCESSION   BBa_J01073
VERSION     BBa_J01073.1  
FEATURES             Location/Qualifiers
     oriT            1..365
                     /label=BBa_J01002
     promoter        411..416
                     /label=-10
     promoter        388..393
                     /label=-35
     misc_feature    398..414
                     /label=OR1
     misc_feature    374..390
                     /label=OR2
     misc_feature    374..422
                     /label=BBa_R0051
     promoter        374..422
                     /label=BBa_R0051
     misc_feature    435..438
                     /label=conserved
     RBS             431..442
                     /label=BBa_B0034
     CDS             449..1123
                     /label=mrfp1
     misc_feature    1130..1154
                     /label=Help:Barcodes
     CDS             449..1154
                     /label=BBa_E1010
     stem_loop       1174..1217
                     /label=stem_loop
     misc_feature    1163..1242
                     /label=BBa_B0010
     terminator      1163..1242
                     /label=BBa_B0010
     stem_loop       1258..1277
                     /label=T7 TE
     misc_feature    1284..1284
                     /label=stop
     polyA_site      1278..1291
                     /label=polya
     misc_feature    1251..1291
                     /label=BBa_B0012
     terminator      1251..1291
                     /label=BBa_B0012
     terminator      1163..1291
                     /label=BBa_B0015
ORIGIN
        1 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaag
       61 tttttgctga tttttcttta taaatagagt gttatgaaaa attagtttct cttactctct
      121 ttatgatatt taaaaaagcg gtgtcggcgc ggctacaaca acgcgccgac accgttttgt
      181 aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt gctgctagcg
      241 gcgcggtgtg tttttttata ggataccgct aggggcgctg ctagcggtgc gtccctgttt
      301 gcattatgaa ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc
      361 taatgtacta gagtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt
      421 gctactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt
      481 catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg
      541 tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg
      601 tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc
      661 ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt
      721 caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc
      781 ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc
      841 cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta
      901 cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg
      961 tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg
     1021 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt
     1081 tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg
     1141 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1201 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1261 tcaccttcgg gtgggccttt ctgcgtttat a
//