LOCUS       BBa_J01074              1297 bp    DNA     linear       27-May-2024
DEFINITION  TEST [OriTR][r0051][StrRBS][RFP][DblTerminator]
ACCESSION   BBa_J01074
VERSION     BBa_J01074.1  
FEATURES             Location/Qualifiers
     oriT            1..371
                     /label=BBa_J01003
     promoter        417..422
                     /label=-10
     promoter        394..399
                     /label=-35
     misc_feature    404..420
                     /label=OR1
     misc_feature    380..396
                     /label=OR2
     misc_feature    380..428
                     /label=BBa_R0051
     promoter        380..428
                     /label=BBa_R0051
     misc_feature    441..444
                     /label=conserved
     RBS             437..448
                     /label=BBa_B0034
     CDS             455..1129
                     /label=mrfp1
     misc_feature    1136..1160
                     /label=Help:Barcodes
     CDS             455..1135
                     /label=BBa_E1010
     stem_loop       1180..1223
                     /label=stem_loop
     misc_feature    1169..1248
                     /label=BBa_B0010
     terminator      1169..1248
                     /label=BBa_B0010
     stem_loop       1264..1283
                     /label=T7 TE
     misc_feature    1290..1290
                     /label=stop
     polyA_site      1284..1297
                     /label=polya
     misc_feature    1257..1297
                     /label=BBa_B0012
     terminator      1257..1297
                     /label=BBa_B0012
ORIGIN
        1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
       61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
      121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
      181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
      241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
      301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
      361 gcagcggaaa atactagagt aacaccgtgc gtgttgacta ttttacctct ggcggtgata
      421 atggttgcta ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa
      481 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat
      541 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac
      601 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc
      661 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga
      721 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca
      781 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt
      841 cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg
      901 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga
      961 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct
     1021 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac
     1081 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg
     1141 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1201 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1261 actggctcac cttcgggtgg gcctttctgc gtttata
//