LOCUS       BBa_J01122               951 bp    DNA     linear       27-May-2024
DEFINITION  [pTet][Lock3][RFP][DblTerm]
ACCESSION   BBa_J01122
VERSION     BBa_J01122.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..102
                     /label=BBa_J01080
     CDS             109..783
                     /label=mrfp1
     misc_feature    790..814
                     /label=Help:Barcodes
     CDS             109..789
                     /label=BBa_E1010
     stem_loop       834..877
                     /label=stem_loop
     misc_feature    823..902
                     /label=BBa_B0010
     terminator      823..902
                     /label=BBa_B0010
     stem_loop       918..937
                     /label=T7 TE
     misc_feature    944..944
                     /label=stop
     polyA_site      938..951
                     /label=polya
     misc_feature    911..951
                     /label=BBa_B0012
     terminator      911..951
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctcttg cttttgggta agaaagagga gatactagat ggcttcctcc
      121 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
      181 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
      241 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
      301 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
      361 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
      421 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
      481 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
      541 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
      601 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
      661 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
      721 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
      781 gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac
      841 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      901 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//