LOCUS BBa_J03103 2935 bp DNA linear 10-May-2024 DEFINITION -- No description -- ACCESSION BBa_J03103 VERSION BBa_J03103.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 230..937 /label=cI lambda misc_feature 938..970 /label=LVA misc_feature 977..1001 /label=Help:Barcodes CDS 227..976 /label=BBa_C0051 stem_loop 1017..1036 /label=T7 TE misc_feature 1043..1043 /label=stop polyA_site 1037..1050 /label=polya misc_feature 1010..1050 /label=BBa_B0012 terminator 1010..1050 /label=BBa_B0012 stem_loop 1071..1093 /label=stem_loop misc_feature 1059..1104 /label=BBa_B0011 terminator 1059..1104 /label=BBa_B0011 misc_feature 1121..1137 /label=OR1 misc_feature 1145..1161 /label=OR2 promoter 1160..1165 /label=-35 misc_feature 1171..1177 /label=mutate to obliterate OR3 promoter 1183..1188 /label=-10 promoter 1113..1194 /label=BBa_I12007 misc_feature 1207..1210 /label=conserved RBS 1203..1214 /label=BBa_B0034 misc_feature 1851..1856 /label=2 CDS 1221..1856 /label=cI 434 CDS 1221..1856 /label=BBa_C0056 stem_loop 1872..1891 /label=T7 TE misc_feature 1898..1898 /label=stop polyA_site 1892..1905 /label=polya misc_feature 1865..1905 /label=BBa_B0012 terminator 1865..1905 /label=BBa_B0012 stem_loop 1926..1948 /label=stem_loop misc_feature 1914..1959 /label=BBa_B0011 terminator 1914..1959 /label=BBa_B0011 misc_feature 2023..2036 /label=OR1 434 misc_feature 1976..1992 /label=OR1 lambda misc_feature 2000..2016 /label=OR2 lambda promoter 2038..2043 /label=-10 promoter 2015..2020 /label=-35 promoter 1968..2049 /label=BBa_I12006 misc_feature 2062..2065 /label=conserved RBS 2058..2069 /label=BBa_B0034 CDS 2076..2798 /label=BBa_E0030 stem_loop 2818..2861 /label=stem_loop misc_feature 2807..2886 /label=BBa_B0010 terminator 2807..2886 /label=BBa_B0010 stem_loop 2902..2921 /label=T7 TE misc_feature 2928..2928 /label=stop polyA_site 2922..2935 /label=polya misc_feature 2895..2935 /label=BBa_B0012 terminator 2895..2935 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa 241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa 301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca 361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt 421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat 481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc 541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg 601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga 661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat 721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact 781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca 841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa 901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc 961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagt cacactggct 1021 caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt 1081 attaatccgg cttttttatt attttactag aggcaaccat tatcaccgcc agaggtaaaa 1141 tagtcaacac gcacggtgtt agatatttat aaatagtggt gatagattta acgttactag 1201 agaaagagga gaaatactag atgagtattt cttccagggt aaaaagcaaa agaatccagc 1261 ttggacttaa ccaggctgaa cttgctcaaa aggtggggac tacccagcag tctatagagc 1321 agctcgaaaa cggtaaaact aagcgaccac gctttttacc agaacttgcg tcagctcttg 1381 gcgtaagtgt tgactggctg ctcaatggca cctctgattc gaatgttaga tttgttgggc 1441 acgttgagcc caaagggaaa tatccattga ttagcatggt tagagctggt tcgtggtgtg 1501 aagcttgtga accctacgat atcaaggaca ttgatgaatg gtatgacagt gacgttaact 1561 tattaggcaa tggattctgg ctgaaggttg aaggtgattc catgacctca cctgtaggtc 1621 aaagcatccc tgaaggtcat atggtgttag tagatactgg acgggagcca gtgaatggaa 1681 gccttgttgt agccaaactg actgacgcga acgaagcaac attcaagaaa ctggtcatag 1741 atggcggtca gaagtacctg aaaggcctga atccttcatg gcctatgact cctatcaacg 1801 gaaactgcaa gattatcggt gttgtcgtgg aagcgagggt aaaattcgta taataatact 1861 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat 1921 ataaaaagcc agattattaa tccggctttt ttattatttt actagaggca accattatca 1981 ccgccagagg taaaatagtc aacacgcacg gtgttagata ttacaaactt tcttgtatag 2041 atttaacgtt actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 2101 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 2161 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 2221 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc 2281 tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca 2341 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 2401 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 2461 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 2521 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 2581 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 2641 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga 2701 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 2761 ggatcactct cggcatggac gagctgtaca agtaataata ctagagccag gcatcaaata 2821 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2881 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //