LOCUS       BBa_J05314               661 bp    DNA     linear       13-Jun-2024
DEFINITION  R4 state
ACCESSION   BBa_J05314
VERSION     BBa_J05314.1  
FEATURES             Location/Qualifiers
     promoter        1..41
                     /label=BBa_J05218
     misc_feature    54..57
                     /label=conserved
     RBS             50..61
                     /label=BBa_B0034
     CDS             89..172
                     /label=Finger 1
     misc_feature    122..145
                     /label=ZF-GGA
     CDS             173..256
                     /label=Finger 2
     misc_feature    206..229
                     /label=ZF-GCC
     CDS             257..340
                     /label=Finger 3
     misc_feature    290..313
                     /label=ZF-GGG
     misc_feature    500..524
                     /label=Help:Barcodes
     CDS             68..524
                     /label=BBa_J05111
     stem_loop       544..587
                     /label=stem_loop
     misc_feature    533..612
                     /label=BBa_B0010
     terminator      533..612
                     /label=BBa_B0010
     stem_loop       628..647
                     /label=T7 TE
     misc_feature    654..654
                     /label=stop
     polyA_site      648..661
                     /label=polya
     misc_feature    621..661
                     /label=BBa_B0012
     terminator      621..661
                     /label=BBa_B0012
     terminator      533..661
                     /label=BBa_B0015
ORIGIN
        1 cgtcccctcc ggaggggacg gccccgcctc cggaggcggg gtactagaga aagaggagaa
       61 atactagatg ctggaaccgg gcgaaaaacc gtataaatgc ccggaatgcg gcaaaagctt
      121 tagccagcgc gcgcatctgg aacgccatca gcgcacccat accggcgaaa aaccgtataa
      181 atgcccggaa tgcggcaaaa gctttagcga ttgccgcgat ctggcgcgcc atcagcgcac
      241 ccataccggc gaaaaaccgt ataaatgccc ggaatgcggc aaaagcttta gccgcagcga
      301 taaactggtg cgccatcagc gcacccatac cggcaaaaaa accagccagc agcgccatgt
      361 gctggaaagc gaaaaaaacc agctgctgca acaggtggat catctgaaac aggaaattag
      421 ccgcctggtg cgcgaacgcg atgcgtataa agaaaaatat gaaaaactgg tgagcagcgg
      481 ctttcgcgaa aactaataac actgatagtg ctagtgtaga tcactactag agccaggcat
      541 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      601 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      661 a
//