LOCUS       BBa_J06007              1691 bp    DNA     linear       20-May-2024
DEFINITION  YFP and LuxI(LVA+) on LuxBox
ACCESSION   BBa_J06007
VERSION     BBa_J06007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..885
                     /label=YFP (LVA)
     misc_feature    880..885
                     /label=2
     misc_feature    841..879
                     /label=SsrA
     misc_feature    365..365
                     /label=A (Q->L)
     misc_feature    124..885
                     /label=BBa_E0032
     misc_feature    124..885
                     /label=BBa_E0032
     misc_feature    898..901
                     /label=conserved
     RBS             894..905
                     /label=BBa_B0034
     misc_feature    1491..1522
                     /label=LVA
     CDS             912..1490
                     /label=luxI
     misc_feature    912..1529
                     /label=BBa_C0061
     misc_feature    1530..1554
                     /label=Help:Barcodes
     CDS             912..1529
                     /label=BBa_C0061
     stem_loop       1574..1617
                     /label=stem_loop
     misc_feature    1563..1642
                     /label=BBa_B0010
     terminator      1563..1642
                     /label=BBa_B0010
     stem_loop       1658..1677
                     /label=T7 TE
     misc_feature    1684..1684
                     /label=stop
     polyA_site      1678..1691
                     /label=polya
     misc_feature    1651..1691
                     /label=BBa_B0012
     terminator      1651..1691
                     /label=BBa_B0012
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      181 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      241 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      301 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg
      361 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      421 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      481 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      541 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      601 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      661 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      721 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
      781 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      841 aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta gagaaagagg
      901 agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg
      961 agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact
     1021 tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt
     1081 atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg
     1141 attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc
     1201 ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact
     1261 ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag
     1321 gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta
     1381 aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg
     1441 ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg
     1501 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag
     1561 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1621 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1681 ctgcgtttat a
//