LOCUS       BBa_J06012               804 bp    DNA     linear       14-May-2024
DEFINITION  Tet repressible luxI generator ( LVA- )
ACCESSION   BBa_J06012
VERSION     BBa_J06012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     CDS             83..667
                     /label=LuxI
     CDS             83..667
                     /label=BBa_C0161
     stem_loop       687..730
                     /label=stem_loop
     misc_feature    676..755
                     /label=BBa_B0010
     terminator      676..755
                     /label=BBa_B0010
     stem_loop       771..790
                     /label=T7 TE
     misc_feature    797..797
                     /label=stop
     polyA_site      791..804
                     /label=polya
     misc_feature    764..804
                     /label=BBa_B0012
     terminator      764..804
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaacctact agatgactat aatgataaaa aaatcggatt ttttggcaat
      121 tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta agcaaagact
      181 tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata actcaaatgc
      241 agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc gtttattacc
      301 tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc
      361 tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa
      421 gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat ataaacacgc
      481 tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag agcgattttt
      541 aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg tattaggtga
      601 tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag cagtcttaaa
      661 ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      721 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      781 cgggtgggcc tttctgcgtt tata
//