LOCUS       BBa_J06014              2098 bp    DNA     linear       13-May-2024
DEFINITION  AHL receiver, CI repressor hybrid
ACCESSION   BBa_J06014
VERSION     BBa_J06014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1173..1176
                     /label=conserved
     RBS             1167..1180
                     /label=BBa_B0031
     CDS             1190..1897
                     /label=cI lambda
     misc_feature    1898..1930
                     /label=LVA
     misc_feature    1937..1961
                     /label=Help:Barcodes
     CDS             1187..1936
                     /label=BBa_C0051
     stem_loop       1981..2024
                     /label=stem_loop
     misc_feature    1970..2049
                     /label=BBa_B0010
     terminator      1970..2049
                     /label=BBa_B0010
     stem_loop       2065..2084
                     /label=T7 TE
     misc_feature    2091..2091
                     /label=stop
     polyA_site      2085..2098
                     /label=polya
     misc_feature    2058..2098
                     /label=BBa_B0012
     terminator      2058..2098
                     /label=BBa_B0012
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagtcac acaggaaacc tactagatga gcacaaaaaa
     1201 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
     1261 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
     1321 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
     1381 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
     1441 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
     1501 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
     1561 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
     1621 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
     1681 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
     1741 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
     1801 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
     1861 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc
     1921 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     1981 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2041 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//