LOCUS       BBa_J06022               803 bp    DNA     linear       12-May-2024
DEFINITION  Tet repressible luxI generator ( LVA- )
ACCESSION   BBa_J06022
VERSION     BBa_J06022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..666
                     /label=LuxI
     CDS             82..666
                     /label=BBa_C0161
     stem_loop       686..729
                     /label=stem_loop
     misc_feature    675..754
                     /label=BBa_B0010
     terminator      675..754
                     /label=BBa_B0010
     stem_loop       770..789
                     /label=T7 TE
     misc_feature    796..796
                     /label=stop
     polyA_site      790..803
                     /label=polya
     misc_feature    763..803
                     /label=BBa_B0012
     terminator      763..803
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      721 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      781 gggtgggcct ttctgcgttt ata
//