LOCUS       BBa_J06027              1692 bp    DNA     linear       13-May-2024
ACCESSION   BBa_J06027
VERSION     BBa_J06027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..885
                     /label=YFP (LVA)
     misc_feature    880..885
                     /label=2
     misc_feature    841..879
                     /label=SsrA
     misc_feature    365..365
                     /label=A (Q->L)
     misc_feature    124..885
                     /label=BBa_E0032
     misc_feature    124..885
                     /label=BBa_E0032
     RBS             894..906
                     /label=RBS-3\Weak
     RBS             900..903
                     /label=RBS
     misc_feature    894..906
                     /label=BBa_B0032
     RBS             894..906
                     /label=BBa_B0032
     misc_feature    1492..1523
                     /label=LVA
     CDS             913..1491
                     /label=luxI
     misc_feature    913..1530
                     /label=BBa_C0061
     misc_feature    1531..1555
                     /label=Help:Barcodes
     CDS             913..1530
                     /label=BBa_C0061
     stem_loop       1575..1618
                     /label=stem_loop
     misc_feature    1564..1643
                     /label=BBa_B0010
     terminator      1564..1643
                     /label=BBa_B0010
     stem_loop       1659..1678
                     /label=T7 TE
     misc_feature    1685..1685
                     /label=stop
     polyA_site      1679..1692
                     /label=polya
     misc_feature    1652..1692
                     /label=BBa_B0012
     terminator      1652..1692
                     /label=BBa_B0012
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      181 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      241 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      301 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg
      361 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      421 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      481 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      541 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      601 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      661 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      721 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
      781 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      841 aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta gagtcacaca
      901 ggaaagtact agatgactat aatgataaaa aaatcggatt ttttggcaat tccatcggag
      961 gagtataaag gtattctaag tcttcgttat caagtgttta agcaaagact tgagtgggac
     1021 ttagttgtag aaaataacct tgaatcagat gagtatgata actcaaatgc agaatatatt
     1081 tatgcttgtg atgatactga aaatgtaagt ggatgctggc gtttattacc tacaacaggt
     1141 gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat
     1201 cctaatatag tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa gataaataac
     1261 tctgctagtg aaattacaat gaaactattt gaagctatat ataaacacgc tgttagtcaa
     1321 ggtattacag aatatgtaac agtaacatca acagcaatag agcgattttt aaagcgtatt
     1381 aaagttcctt gtcatcgtat tggagacaaa gaaattcatg tattaggtga tactaaatcg
     1441 gttgtattgt ctatgcctat taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac
     1501 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta
     1561 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1621 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1681 tctgcgttta ta
//