LOCUS       BBa_J06100              2072 bp    DNA     linear       10-May-2024
DEFINITION  3OC12HSL to YFP receiver test construct
ACCESSION   BBa_J06100
VERSION     BBa_J06100.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     CDS             65..781
                     /label=lasR
     misc_feature    782..814
                     /label=LVA
     misc_feature    544..544
                     /label=G
     misc_feature    821..845
                     /label=Help:Barcodes
     CDS             65..820
                     /label=BBa_C0079
     stem_loop       865..908
                     /label=stem_loop
     misc_feature    854..933
                     /label=BBa_B0010
     terminator      854..933
                     /label=BBa_B0010
     stem_loop       949..968
                     /label=T7 TE
     misc_feature    975..975
                     /label=stop
     polyA_site      969..982
                     /label=polya
     misc_feature    942..982
                     /label=BBa_B0012
     terminator      942..982
                     /label=BBa_B0012
     misc_feature    1036..1053
                     /label=OP2
     misc_feature    1096..1115
                     /label=OP1
     promoter        1107..1112
                     /label=-35
     promoter        1130..1135
                     /label=-10
     promoter        991..1147
                     /label=BBa_R0079
     misc_feature    1160..1163
                     /label=conserved
     RBS             1156..1167
                     /label=BBa_B0034
     CDS             1174..1935
                     /label=YFP (LVA)
     misc_feature    1930..1935
                     /label=2
     misc_feature    1891..1929
                     /label=SsrA
     misc_feature    1415..1415
                     /label=A (Q->L)
     misc_feature    1174..1935
                     /label=BBa_E0032
     misc_feature    1174..1935
                     /label=BBa_E0032
     stem_loop       1955..1998
                     /label=stem_loop
     misc_feature    1944..2023
                     /label=BBa_B0010
     terminator      1944..2023
                     /label=BBa_B0010
     stem_loop       2039..2058
                     /label=T7 TE
     misc_feature    2065..2065
                     /label=stop
     polyA_site      2059..2072
                     /label=polya
     misc_feature    2032..2072
                     /label=BBa_B0012
     terminator      2032..2072
                     /label=BBa_B0012
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatggcc ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg
      121 gagcgccatc ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct
      181 gttgcctaag gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc
      241 ctggcgcgag cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg
      301 tacccagagc gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca
      361 cgagttcttc gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca
      421 tggtgctcgc ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga
      481 ggccaaccgt ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact
      541 gcaaagcggt gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag
      601 ccgggagaag gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt
      661 tatctgcaac tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt
      721 cggtgtgacc tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct
      781 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag
      841 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta tatactagag gcccctcgct gagcgcgtcc cggagctggg
     1021 ggcaacctag ctgccacctg cttttctgct agctattcca gcgaaaacat acagatttcc
     1081 ggcgaaatca aggctacctg ccagttctgg caggtttggc cgcgggttct ttttggtaca
     1141 cgaaagctac tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc
     1201 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1261 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1321 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     1381 caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg
     1441 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1501 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1561 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1621 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1681 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1741 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     1801 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1861 atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct
     1921 ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1981 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2041 ctcaccttcg ggtgggcctt tctgcgttta ta
//