LOCUS       BBa_J06108              1728 bp    DNA     linear       06-May-2024
DEFINITION  YFP and LasI(LVA-) on LasBox
ACCESSION   BBa_J06108
VERSION     BBa_J06108.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     misc_feature    181..184
                     /label=conserved
     RBS             177..188
                     /label=BBa_B0034
     misc_feature    951..956
                     /label=stop
     misc_feature    912..950
                     /label=AAV
     misc_feature    195..956
                     /label=BBa_E0034
     misc_feature    969..972
                     /label=conserved
     RBS             965..976
                     /label=BBa_B0034
     CDS             983..1591
                     /label=lasI
     CDS             983..1591
                     /label=BBa_C0178
     stem_loop       1611..1654
                     /label=stem_loop
     misc_feature    1600..1679
                     /label=BBa_B0010
     terminator      1600..1679
                     /label=BBa_B0010
     stem_loop       1695..1714
                     /label=T7 TE
     misc_feature    1721..1721
                     /label=stop
     polyA_site      1715..1728
                     /label=polya
     misc_feature    1688..1728
                     /label=BBa_B0012
     terminator      1688..1728
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagaaag
      181 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      361 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
      421 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      661 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      781 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
      841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      901 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tgctgctgtt taataatact
      961 agagaaagag gagaaatact agatgatcgt tcagatcggt cgtcgtgaag agttcgacaa
     1021 aaaactgctg ggtgaaatgc acaaactgcg tgctcaggtt ttcaaagaac gtaaaggttg
     1081 ggacgtttcc gttatcgacg aaatggaaat cgacggttac gacgctctgt ccccgtacta
     1141 catgctgatc caggaagaca ccccggaagc tcaggttttc ggttgctggc gtatcttcga
     1201 caccaccggt ccgtacatgc tgaaaaacac cttcccggaa ctgctgcacg gtaaagaagc
     1261 tccgtgctcc ccgcacatct gggaactgtc ccgtttcgct atcaactccg gtcagaaagg
     1321 ttccctgggt ttctccgact gcaccctgga agctatgcgt gctctggctc gttactcctt
     1381 gcagaacgac atccagaccc tggttaccgt taccaccgtt ggtgttgaaa aaatgatgat
     1441 ccgtgctggt ctggacgttt cccgtttcgg tccgcacctg aaaatcggta tcgaacgtgc
     1501 tgttgctctg cgtatcgaac tgaacgctaa aacccagatc gctctgtacg gtggtgttct
     1561 ggttgaacag cgtctggctg tttcctaata atactagagc caggcatcaa ataaaacgaa
     1621 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1681 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//