LOCUS       BBa_J06112               828 bp    DNA     linear       12-May-2024
ACCESSION   BBa_J06112
VERSION     BBa_J06112.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     CDS             83..691
                     /label=lasI
     CDS             83..691
                     /label=BBa_C0178
     stem_loop       711..754
                     /label=stem_loop
     misc_feature    700..779
                     /label=BBa_B0010
     terminator      700..779
                     /label=BBa_B0010
     stem_loop       795..814
                     /label=T7 TE
     misc_feature    821..821
                     /label=stop
     polyA_site      815..828
                     /label=polya
     misc_feature    788..828
                     /label=BBa_B0012
     terminator      788..828
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaacctact agatgatcgt tcagatcggt cgtcgtgaag agttcgacaa
      121 aaaactgctg ggtgaaatgc acaaactgcg tgctcaggtt ttcaaagaac gtaaaggttg
      181 ggacgtttcc gttatcgacg aaatggaaat cgacggttac gacgctctgt ccccgtacta
      241 catgctgatc caggaagaca ccccggaagc tcaggttttc ggttgctggc gtatcttcga
      301 caccaccggt ccgtacatgc tgaaaaacac cttcccggaa ctgctgcacg gtaaagaagc
      361 tccgtgctcc ccgcacatct gggaactgtc ccgtttcgct atcaactccg gtcagaaagg
      421 ttccctgggt ttctccgact gcaccctgga agctatgcgt gctctggctc gttactcctt
      481 gcagaacgac atccagaccc tggttaccgt taccaccgtt ggtgttgaaa aaatgatgat
      541 ccgtgctggt ctggacgttt cccgtttcgg tccgcacctg aaaatcggta tcgaacgtgc
      601 tgttgctctg cgtatcgaac tgaacgctaa aacccagatc gctctgtacg gtggtgttct
      661 ggttgaacag cgtctggctg tttcctaata atactagagc caggcatcaa ataaaacgaa
      721 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      781 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//