LOCUS       BBa_J06118              1730 bp    DNA     linear       11-May-2024
ACCESSION   BBa_J06118
VERSION     BBa_J06118.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     misc_feature    181..184
                     /label=conserved
     RBS             177..188
                     /label=BBa_B0034
     CDS             195..956
                     /label=YFP (LVA)
     misc_feature    951..956
                     /label=2
     misc_feature    912..950
                     /label=SsrA
     misc_feature    436..436
                     /label=A (Q->L)
     misc_feature    195..956
                     /label=BBa_E0032
     misc_feature    195..956
                     /label=BBa_E0032
     misc_feature    971..974
                     /label=conserved
     RBS             965..978
                     /label=BBa_B0031
     CDS             985..1593
                     /label=lasI
     CDS             985..1593
                     /label=BBa_C0178
     stem_loop       1613..1656
                     /label=stem_loop
     misc_feature    1602..1681
                     /label=BBa_B0010
     terminator      1602..1681
                     /label=BBa_B0010
     stem_loop       1697..1716
                     /label=T7 TE
     misc_feature    1723..1723
                     /label=stop
     polyA_site      1717..1730
                     /label=polya
     misc_feature    1690..1730
                     /label=BBa_B0012
     terminator      1690..1730
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagaaag
      181 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      361 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
      421 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      661 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      781 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
      841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      901 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact
      961 agagtcacac aggaaaccta ctagatgatc gttcagatcg gtcgtcgtga agagttcgac
     1021 aaaaaactgc tgggtgaaat gcacaaactg cgtgctcagg ttttcaaaga acgtaaaggt
     1081 tgggacgttt ccgttatcga cgaaatggaa atcgacggtt acgacgctct gtccccgtac
     1141 tacatgctga tccaggaaga caccccggaa gctcaggttt tcggttgctg gcgtatcttc
     1201 gacaccaccg gtccgtacat gctgaaaaac accttcccgg aactgctgca cggtaaagaa
     1261 gctccgtgct ccccgcacat ctgggaactg tcccgtttcg ctatcaactc cggtcagaaa
     1321 ggttccctgg gtttctccga ctgcaccctg gaagctatgc gtgctctggc tcgttactcc
     1381 ttgcagaacg acatccagac cctggttacc gttaccaccg ttggtgttga aaaaatgatg
     1441 atccgtgctg gtctggacgt ttcccgtttc ggtccgcacc tgaaaatcgg tatcgaacgt
     1501 gctgttgctc tgcgtatcga actgaacgct aaaacccaga tcgctctgta cggtggtgtt
     1561 ctggttgaac agcgtctggc tgtttcctaa taatactaga gccaggcatc aaataaaacg
     1621 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1681 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//