LOCUS       BBa_J06122               827 bp    DNA     linear       12-May-2024
ACCESSION   BBa_J06122
VERSION     BBa_J06122.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..690
                     /label=lasI
     CDS             82..690
                     /label=BBa_C0178
     stem_loop       710..753
                     /label=stem_loop
     misc_feature    699..778
                     /label=BBa_B0010
     terminator      699..778
                     /label=BBa_B0010
     stem_loop       794..813
                     /label=T7 TE
     misc_feature    820..820
                     /label=stop
     polyA_site      814..827
                     /label=polya
     misc_feature    787..827
                     /label=BBa_B0012
     terminator      787..827
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgatcgtt cagatcggtc gtcgtgaaga gttcgacaaa
      121 aaactgctgg gtgaaatgca caaactgcgt gctcaggttt tcaaagaacg taaaggttgg
      181 gacgtttccg ttatcgacga aatggaaatc gacggttacg acgctctgtc cccgtactac
      241 atgctgatcc aggaagacac cccggaagct caggttttcg gttgctggcg tatcttcgac
      301 accaccggtc cgtacatgct gaaaaacacc ttcccggaac tgctgcacgg taaagaagct
      361 ccgtgctccc cgcacatctg ggaactgtcc cgtttcgcta tcaactccgg tcagaaaggt
      421 tccctgggtt tctccgactg caccctggaa gctatgcgtg ctctggctcg ttactccttg
      481 cagaacgaca tccagaccct ggttaccgtt accaccgttg gtgttgaaaa aatgatgatc
      541 cgtgctggtc tggacgtttc ccgtttcggt ccgcacctga aaatcggtat cgaacgtgct
      601 gttgctctgc gtatcgaact gaacgctaaa acccagatcg ctctgtacgg tggtgttctg
      661 gttgaacagc gtctggctgt ttcctaataa tactagagcc aggcatcaaa taaaacgaaa
      721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      781 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//