LOCUS       BBa_J06127              1790 bp    DNA     linear       10-May-2024
ACCESSION   BBa_J06127
VERSION     BBa_J06127.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     RBS             177..191
                     /label=RBS-1\Strong
     RBS             184..188
                     /label=RBS
     misc_feature    177..191
                     /label=BBa_B0030
     RBS             177..191
                     /label=BBa_B0030
     CDS             198..959
                     /label=YFP (LVA)
     misc_feature    954..959
                     /label=2
     misc_feature    915..953
                     /label=SsrA
     misc_feature    439..439
                     /label=A (Q->L)
     misc_feature    198..959
                     /label=BBa_E0032
     misc_feature    198..959
                     /label=BBa_E0032
     RBS             968..980
                     /label=RBS-3\Weak
     RBS             974..977
                     /label=RBS
     misc_feature    968..980
                     /label=BBa_B0032
     RBS             968..980
                     /label=BBa_B0032
     CDS             987..1589
                     /label=LasI
     misc_feature    1590..1622
                     /label=LVA
     misc_feature    1629..1653
                     /label=Help:Barcodes
     CDS             987..1628
                     /label=BBa_C0078
     stem_loop       1673..1716
                     /label=stem_loop
     misc_feature    1662..1741
                     /label=BBa_B0010
     terminator      1662..1741
                     /label=BBa_B0010
     stem_loop       1757..1776
                     /label=T7 TE
     misc_feature    1783..1783
                     /label=stop
     polyA_site      1777..1790
                     /label=polya
     misc_feature    1750..1790
                     /label=BBa_B0012
     terminator      1750..1790
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagatta
      181 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      241 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      301 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      361 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      421 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      481 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      541 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      601 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      661 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      721 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      781 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      841 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      901 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      961 actagagtca cacaggaaag tactagatga tcgttcagat cggtcgtcgt gaagagttcg
     1021 acaaaaaact gctgggtgaa atgcacaaac tgcgtgctca ggttttcaaa gaacgtaaag
     1081 gttgggacgt ttccgttatc gacgaaatgg aaatcgacgg ttacgacgct ctgtccccgt
     1141 actacatgct gatccaggaa gacaccccgg aagctcaggt tttcggttgc tggcgtatct
     1201 tcgacaccac cggtccgtac atgctgaaaa acaccttccc ggaactgctg cacggtaaag
     1261 aagctccgtg ctccccgcac atctgggaac tgtcccgttt cgctatcaac tccggtcaga
     1321 aaggttccct gggtttctcc gactgcaccc tggaagctat gcgtgctctg gctcgttact
     1381 ccttgcagaa cgacatccag accctggtta ccgttaccac cgttggtgtt gaaaaaatga
     1441 tgatccgtgc tggtctggac gtttcccgtt tcggtccgca cctgaaaatc ggtatcgaac
     1501 gtgctgttgc tctgcgtatc gaactgaacg ctaaaaccca gatcgctctg tacggtggtg
     1561 ttctggttga acagcgtctg gctgtttccg ctgctaacga cgaaaactac gctctggttg
     1621 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1741 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//