LOCUS       BBa_J06128              1729 bp    DNA     linear       13-May-2024
ACCESSION   BBa_J06128
VERSION     BBa_J06128.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     misc_feature    181..184
                     /label=conserved
     RBS             177..188
                     /label=BBa_B0034
     CDS             195..956
                     /label=YFP (LVA)
     misc_feature    951..956
                     /label=2
     misc_feature    912..950
                     /label=SsrA
     misc_feature    436..436
                     /label=A (Q->L)
     misc_feature    195..956
                     /label=BBa_E0032
     misc_feature    195..956
                     /label=BBa_E0032
     RBS             965..977
                     /label=RBS-3\Weak
     RBS             971..974
                     /label=RBS
     misc_feature    965..977
                     /label=BBa_B0032
     RBS             965..977
                     /label=BBa_B0032
     CDS             984..1592
                     /label=lasI
     CDS             984..1592
                     /label=BBa_C0178
     stem_loop       1612..1655
                     /label=stem_loop
     misc_feature    1601..1680
                     /label=BBa_B0010
     terminator      1601..1680
                     /label=BBa_B0010
     stem_loop       1696..1715
                     /label=T7 TE
     misc_feature    1722..1722
                     /label=stop
     polyA_site      1716..1729
                     /label=polya
     misc_feature    1689..1729
                     /label=BBa_B0012
     terminator      1689..1729
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagaaag
      181 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      361 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
      421 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      661 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      781 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
      841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      901 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact
      961 agagtcacac aggaaagtac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca
     1021 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt
     1081 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact
     1141 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg
     1201 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag
     1261 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag
     1321 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct
     1381 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga
     1441 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg
     1501 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc
     1561 tggttgaaca gcgtctggct gtttcctaat aatactagag ccaggcatca aataaaacga
     1621 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1681 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//